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  • Featured Product
  • KD/KO Validated

PIR Polyclonal antibody

PIR Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 10263-1-AP

Print datasheet

Synonyms

PIR, Pirin

验证数据展示

  • WB analysis of HepG2 using 10263-1-AP

    WB analysis of HepG2 using 10263-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10263-1-AP (PIR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 10263-1-AP

    IP experiment of HeLa using 10263-1-AP

    IP result of anti-PIR (IP:10263-1-AP, 4ug; Detection:10263-1-AP 1:300) with HeLa cells lysate 1200ug.

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10263-1-AP targets PIR in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen PIR fusion protein Ag0330 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pirin (iron-binding nuclear protein)
Synonyms PIR, Pirin
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC002517
Gene Symbol PIR
Gene ID (NCBI) 8544
RRIDAB_2283879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00625
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pirin (iron-binding nuclear protein, PIR) is a highly conserved 32-kDa protein consisting of 290 amino acids. Pirin mRNA is expressed weakly in all human tissues. Confocal immunofluorescence experiments demonstrate a predominant localization of Pirin within dot-like subnuclear structures. Pirin interacts with the oncoprotein B-cell lymphoma 3-encoded (Bcl-3) and nuclear factor I (NFI).

实验方案

Product Specific Protocols
WB protocol for PIR antibody 10263-1-APDownload protocol
IP protocol for PIR antibody 10263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Cycle

PIR promotes tumorigenesis of breast cancer by upregulating cell cycle activator E2F1.

Authors - Muhammad Suleman
  • KD Validated
View Article