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验证数据展示

  • WB analysis of HEK-293 using 17547-1-AP

    WB analysis of HEK-293 using 17547-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17547-1-AP (PIP5KL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 17547-1-AP

    IF Staining of HEK-293 using 17547-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 17547-1-AP (PIP5KL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PIP5KL1 Polyclonal antibody

PIP5KL1 Polyclonal Antibody for WB, IF, ELISA
Cat No. 17547-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF, ELISA

别名

PI(4)P 5 kinase like protein 1, PIP5KL1, PIPKH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, human brain tissue
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17547-1-AP targets PIP5KL1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11653

Product name: Recombinant human PIP5KL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-191 aa of BC042184

Sequence: MQSVFYPAGRISERYDIKGCEVSRWVDPAPEGSPLVLVLKDLNFQGKTINLGPQRSWFLRQMELDTTFLRELNVLDYSLLIAFQRLHEDERGPGSSLIFRTARSVQGAQSPEESRAQNRRLLPDAPNALHILDGPEQRYFLGVVDLATVYGLRKRLEHLWKTLRYPGRTFSTVSPARYARRLCQWVEAHTE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatidylinositol-4-phosphate 5-kinase-like 1
别名 PI(4)P 5 kinase like protein 1, PIP5KL1, PIPKH
计算分子量 22 kDa, 44 kDa
观测分子量 45 kDa
GenBank蛋白编号BC042184
基因名称 PIP5KL1
Gene ID (NCBI) 138429
RRIDAB_2252440
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T9C9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIP5KL1 antibody 17547-1-APDownload protocol
IF protocol for PIP5KL1 antibody 17547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols