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验证数据展示

  • WB analysis of A549 using 27640-1-AP

    WB analysis of A549 using 27640-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 27640-1-AP (PIP5K1C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 27640-1-AP

    IHC staining of human liver cancer using 27640-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27640-1-AP (PIP5K1C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 27640-1-AP

    IHC staining of human liver cancer using 27640-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27640-1-AP (PIP5K1C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PIP5K1C Polyclonal antibody

PIP5K1C Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27640-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

KIAA0589, LCCS3, PIP5K GAMMA, PIP5K1 gamma, PIP5Kgamma

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27640-1-AP targets PIP5K1C in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag26525

Product name: Recombinant human PIP5K1C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 480-573 aa of NM_001195733

Sequence: MIPSEREEAQYDLRGARSYPTLEDEGRPDLLPCTPPSFEEATTASIATTLSSTSLSIPERSPSETSEQPRYRRRTQSSGQDGRPQEEPPAEEDLQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatidylinositol-4-phosphate 5-kinase, type I, gamma
别名 KIAA0589, LCCS3, PIP5K GAMMA, PIP5K1 gamma, PIP5Kgamma
计算分子量 73 kDa
观测分子量 70 kDa
GenBank蛋白编号NM_001195733
基因名称 PIP5K1C
Gene ID (NCBI) 23396
RRIDAB_2880936
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60331
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIP5K1C antibody 27640-1-APDownload protocol
IHC protocol for PIP5K1C antibody 27640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

EBioMedicine

Type Iγ phosphatidylinositol phosphate kinase promotes tumor growth by facilitating Warburg effect in colorectal cancer.

Authors - Wei Peng
View Article

Cell Signal

The miR-424-3p/PIP5K1C axis regulates AKT phosphorylation and granulosa cell proliferation.

Authors - Shuaixiang Mao
View Article
humanWB,IHC

J Immunol Res

PIPKIγ Regulates CCL2 Expression in Colorectal Cancer by Activating AKT-STAT3 Signaling.

Authors - JunLi Xue
View Article
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