验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue, human brain tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human lung tissue, human heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
12541-1-AP targets PIP5K1B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3261 Product name: Recombinant human PIP5K1B protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 107-405 aa of BC030587 Sequence: SEGSNLTPAHHYPDFRFKTYAPLAFRYFRELFGIKPDDYLYSICSEPLIELSNPGASGSLFFVTSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCMQSGGINIRIVVMNNVLPRSMRMHFTYDLKGSTYKRRASRKEREKSNPTFKDLDFLQDMHEGLYFDTETYNALMKTLQRDCRVLESFKIMDYSLLLGIHFLDHSLKEKEEETPQNVPDAKRTGMQKVLYSTAMESIQGPGKSGDGIITENPDTMGGIPAKSHRGEKLLLFTGIIDILQSYRLMKKLEHSW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphatidylinositol-4-phosphate 5-kinase, type I, beta |
| 别名 | PIP5K1 beta, PIP5K1B, PIP5KIbeta, Protein STM 7, PtdIns(4)P 5 kinase 1 beta, STM7 |
| 计算分子量 | 549 aa, 62 kDa |
| 观测分子量 | 62 kDa |
| GenBank蛋白编号 | BC030587 |
| 基因名称 | PIP5K1B |
| Gene ID (NCBI) | 8395 |
| RRID | AB_10734598 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14986 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PIP5K1B antibody 12541-1-AP | Download protocol |
| IP protocol for PIP5K1B antibody 12541-1-AP | Download protocol |
| WB protocol for PIP5K1B antibody 12541-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Spastic paraplegia proteins spastizin and spatacsin mediate autophagic lysosome reformation. | ||
EMBO J PTPRN2 and PLCβ1 promote metastatic breast cancer cell migration through PI(4,5)P2-dependent actin remodeling. | ||
EMBO J Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKHM1 membrane cycling during autophagosome-lysosome fusion.
| ||
Cell Chem Biol Targeting the PI5P4K Lipid Kinase Family in Cancer Using Covalent Inhibitors. | ||
EMBO J Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKMH1 membrane cycling during autophagosome-lysosome fusion. |
