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验证数据展示

  • WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

    WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67483-1-Ig (same clone as 67483-1-PBS)

    IHC staining of human tonsillitis using 67483-1-Ig (same clone as 67483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67483-1-Ig (PIP5K1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIP5K1A antibody included
  • IHC staining of human tonsillitis using 67483-1-Ig (same clone as 67483-1-PBS)

    IHC staining of human tonsillitis using 67483-1-Ig (same clone as 67483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67483-1-Ig (PIP5K1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIP5K1A antibody included
  • WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

    WB analysis using 67483-1-Ig (same clone as 67483-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67483-1-Ig (PIP5K1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67483-1-PBS in a different storage buffer formulation.

PIP5K1A Monoclonal antibody, PBS Only

PIP5K1A Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67483-1-PBS

产品说明书

CloneNo. 3E5G12

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IHC, Indirect ELISA

别名

PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PIP5K1A Ready-To-Use IHC Kit. PIP5K1A primary antibody included.

产品信息

67483-1-PBS targets PIP5K1A in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag8355

Product name: Recombinant human PIP5K1A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 151-500 aa of BC007833

Sequence: SLCSEPLIELCSSGASGSLFYVSSDDEFIIKTVQHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCVQAGGKNIRIVVMNNLLPRSVKMHIKYDLKGSTYKRRASQKEREKPLPTFKDLDFLQDIPDGLFLDADMYNALCKTLQRDCLVLQSFKIMDYSLLMSIHNIDHAQREPLSSETQYSVDTRRPAPQKALYSTAMESIQGEARRGGTMETDDHMGGIPARNSKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVHRPGFYAERFQRFMCNTVFKKIPCVHLGRPDVLPQTPPLEEISEGSPIPDPSFSPLVGETLQMLTTSTTLEKLEVAESEFTH

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
别名 PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha
计算分子量 56 kDa, 63 kDa
观测分子量 63 kDa
GenBank蛋白编号BC007833
基因名称 PIP5K1A
Gene ID (NCBI) 8394
RRIDAB_2882710
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ99755
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa.

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