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验证数据展示

  • WB analysis using 17077-1-AP

    WB analysis using 17077-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 17077-1-AP

    IHC staining of human liver cancer using 17077-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17077-1-AP (PIP4K2C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIP4K2C antibody included
  • IHC staining of human liver cancer using 17077-1-AP

    IHC staining of human liver cancer using 17077-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17077-1-AP (PIP4K2C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PIP4K2C antibody included
  • WB analysis using 17077-1-AP

    WB analysis using 17077-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 17077-1-AP

    WB analysis using 17077-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 17077-1-AP

    WB analysis using 17077-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17077-1-AP (PIP4K2C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

PIP4K2C Polyclonal antibody

PIP4K2C Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17077-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

EC:2.7.1.149, Phosphatidylinositol 5-phosphate 4-kinase type II gamma, Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma, PI(5)P 4-kinase type II gamma, PIP4KII gamma

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, PC-12 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PIP4K2C Ready-To-Use IHC Kit. PIP4K2C primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17077-1-AP targets PIP4K2C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag10989

Product name: Recombinant human PIP4K2C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-421 aa of BC028596

Sequence: MASSSVPPATVSAATAGPGPGFGFASKTKKKHFVQQKVKVFRAADPLVGVFLWGVAHSINELSQVPPPVMLLPDDFKASSKIKVNNHLFHRENLPSHFKFKEYCPQVFRNLRDRFGIDDQDYLVSLTRNPPSESEGSDGRFLISYDRTLVIKEVSSEDIADMHSNLSNYHQYIVKCHGNTLLPQFLGMYRVSVDNEDSYMLVMRNMFSHRLPVHRKYDLKGSLVSREASDKEKVKELPTLRDMDFLNKNQKVYIGEEEKKIFLEKLKRDVEFLVQLKIMDYSLLLGIHDIIRGSEPEEEAPVREDESEVDGDCSLTGPPALVGSYGTSPEGIGGYIHSHRPLGPGEFESFIDVYAIRSAEGAPQKEVYFMGLIDILTQYDAKKKAAHAAKTVKHGAGAEISTVHPEQYAKRFLDFITNIFA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatidylinositol-5-phosphate 4-kinase, type II, gamma
别名 EC:2.7.1.149, Phosphatidylinositol 5-phosphate 4-kinase type II gamma, Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma, PI(5)P 4-kinase type II gamma, PIP4KII gamma
计算分子量 421 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC028596
基因名称 PIP4K2C
Gene ID (NCBI) 79837
RRIDAB_2715526
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TBX8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIP4K2C is involved in several processes, including 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process; negative regulation of 1-phosphatidylinositol-4-phosphate 5-kinase activity; and positive regulation of autophagosome assembly. PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism.

实验方案

Product Specific Protocols
IHC protocol for PIP4K2C antibody 17077-1-APDownload protocol
WB protocol for PIP4K2C antibody 17077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Elife

Inhibition of PIP4Kγ ameliorates the pathological effects of mutant huntingtin protein.

Authors - Ismael Al-Ramahi
  • KD Validated
View Article
humanWB

Cell Rep

PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent Mechanism.

Authors - Diana G Wang
  • KO Validated
View Article
humanWB

Autophagy Rep

Upregulation of the ESCRT pathway and multivesicular bodies accelerates degradation of proteins associated with neurodegeneration

Authors - Ron Benyair
View Article
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