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验证数据展示

  • WB analysis of mouse brain using 12469-1-AP (same clone as 12469-1-PBS)

    WB analysis of mouse brain using 12469-1-AP (same clone as 12469-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 12469-1-AP (same clone as 12469-1-PBS)

    IP experiment of mouse brain using 12469-1-AP (same clone as 12469-1-PBS)

    IP result of anti-PIP4K2A (IP:12469-1-AP, 4ug; Detection:12469-1-AP 1:500) with mouse brain tissue lysate 4400ug. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

  • IHC staining of human spleen using 12469-1-AP (same clone as 12469-1-PBS)

    IHC staining of human spleen using 12469-1-AP (same clone as 12469-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIP4K2A antibody included
  • IHC staining of human spleen using 12469-1-AP (same clone as 12469-1-PBS)

    IHC staining of human spleen using 12469-1-AP (same clone as 12469-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIP4K2A antibody included
  • IF Staining of HepG2 using 12469-1-AP (same clone as 12469-1-PBS)

    IF Staining of HepG2 using 12469-1-AP (same clone as 12469-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PIP4K2A antibody (12469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (black). This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 12469-1-AP (same clone as 12469-1-PBS)

    WB analysis of mouse brain using 12469-1-AP (same clone as 12469-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

  • WB analysis using 12469-1-AP (same clone as 12469-1-PBS)

    WB analysis using 12469-1-AP (same clone as 12469-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

  • WB analysis using 12469-1-AP (same clone as 12469-1-PBS)

    WB analysis using 12469-1-AP (same clone as 12469-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 12469-1-AP (same clone as 12469-1-PBS)

    IF Staining of SH-SY5Y using 12469-1-AP (same clone as 12469-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 12469-1-AP (PIP4K2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12469-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PIP4K2A Polyclonal antibody, PBS Only

PIP4K2A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12469-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha, Phosphatidylinositol 5-phosphate 4-kinase type II alpha, Phosphatidylinositol 5-Phosphate 4-Kinase, EC:2.7.1.149, Diphosphoinositide kinase 2-alpha

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PIP4K2A Ready-To-Use IHC Kit. PIP4K2A primary antibody included.

产品信息

12469-1-PBS targets PIP4K2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3152

Product name: Recombinant human PIP4K2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 59-406 aa of BC018034

Sequence: VMLMPDDFKAYSKIKVDNHLFNKENMPSHFKFKEYCPMVFRNLRERFGIDDQDFQNSLTRSAPLPNDSQARSGARFHTSYDKRYIIKTITSEDVAEMHNILKKYHQYIVECHGITLLPQFLGMYRLNVDGVEIYVIVTRNVFSHRLSVYRKYDLKGSTVAREASDKEKAKELPTLKDNDFINEGQKIYIDDNNKKVFLEKLKKDVEFLAQLKLMDYSLLVGIHDVERAEQEEVECEENDGEEEGESDGTHPVGTPPDSPGNTLNSSPPLAPGEFDPNIDVYGIKCHENSPRKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFLDFIGHILT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
别名 Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha, Phosphatidylinositol 5-phosphate 4-kinase type II alpha, Phosphatidylinositol 5-Phosphate 4-Kinase, EC:2.7.1.149, Diphosphoinositide kinase 2-alpha
计算分子量 406 aa, 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC018034
基因名称 PIP4K2A
Gene ID (NCBI) 5305
RRIDAB_10639518
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48426
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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