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GCDFP-15/PIP Polyclonal antibody

GCDFP-15/PIP Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC,ELISA

Conjugate

Unconjugated

Cat No : 16068-1-AP

Print datasheet

Synonyms

GCDFP 15, GCDFP15, gp17, GPIP4, PIP, prolactin induced protein, Prolactin inducible protein, SABP

验证数据展示

  • IHC staining of human breast cancer using 16068-1-AP

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 16068-1-AP

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman breast cancer tissue, human skin tissue, human breast tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16068-1-AP targets GCDFP-15/PIP in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC,ELISA
Tested Reactivity human
Immunogen GCDFP-15/PIP fusion protein Ag9004 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prolactin-induced protein
Synonyms GCDFP 15, GCDFP15, gp17, GPIP4, PIP, prolactin induced protein, Prolactin inducible protein, SABP
Calculated Molecular Weight 146 aa, 17 kDa
GenBank Accession NumberBC010950
Gene Symbol PIP
Gene ID (NCBI) 5304
RRIDAB_2878212
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12273
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GCDFP-15 (gross cystic disease fluid protein 15), also known as PIP (prolactin-induced protein) is a secretory glycoprotein expressed in benign and malignant breast tumor tissues and in some normal exocrine organs such as sweat, salivary, and lacrimal glands (PMID: 12393800). GCDFP-15 expression is increased by prolactin and androgen and inhibited by estrogen (PMID: 18854942). The expression is also regulated by interleukins. GCDFP-15 is a marker of apocrine differentiation and is frequently used for assessment of metastases or regional recurrences of breast origin.

实验方案

Product Specific Protocols
IHC protocol for GCDFP-15/PIP antibody 16068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols