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验证数据展示

  • IHC staining of human breast cancer using 16068-1-AP

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 16068-1-AP

    IHC staining of human breast cancer using 16068-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GCDFP-15/PIP Polyclonal antibody

GCDFP-15/PIP Polyclonal Antibody for IHC, ELISA
Cat No. 16068-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

GCDFP 15, GCDFP15, gp17, GPIP4, PIP, prolactin induced protein, Prolactin inducible protein, SABP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman breast cancer tissue, human skin tissue, human breast tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16068-1-AP targets GCDFP-15/PIP in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9004

Product name: Recombinant human GCDFP-15, PIP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 28-146 aa of BC010950

Sequence: AQDNTRKIIIKNFDIPKSVRPNDEVTAVLAVQTELKECMVVKTYLISSIPLQGAFNYKYTACLCDDNPKTFYWDFYTNRTVQIAAVVDVIRELGICPDDAAVIPIKNNRFYTIEILKVE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 prolactin-induced protein
别名 GCDFP 15, GCDFP15, gp17, GPIP4, PIP, prolactin induced protein, Prolactin inducible protein, SABP
计算分子量 146 aa, 17 kDa
GenBank蛋白编号BC010950
基因名称 GCDFP-15
Gene ID (NCBI) 5304
RRIDAB_2878212
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12273
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GCDFP-15 (gross cystic disease fluid protein 15), also known as PIP (prolactin-induced protein) is a secretory glycoprotein expressed in benign and malignant breast tumor tissues and in some normal exocrine organs such as sweat, salivary, and lacrimal glands (PMID: 12393800). GCDFP-15 expression is increased by prolactin and androgen and inhibited by estrogen (PMID: 18854942). The expression is also regulated by interleukins. GCDFP-15 is a marker of apocrine differentiation and is frequently used for assessment of metastases or regional recurrences of breast origin.

实验方案

Product Specific Protocols
IHC protocol for GCDFP-15/PIP antibody 16068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols