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验证数据展示

  • WB analysis using 81991-4-RR (same clone as 81991-4-PBS)

    WB analysis using 81991-4-RR (same clone as 81991-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81991-4-RR (PINK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81991-4-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 81991-4-RR (same clone as 81991-4-PBS)

    FC experiment of Jurkat using 81991-4-RR (same clone as 81991-4-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PINK1 Recombinant antibody (81991-4-RR, Clone:240449E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 81991-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81991-4-RR

    Affinity and Kinetic Characterization of 81991-4-RR

    Biolayer interferometry (BLl) kinetic assays of 81991-4-RR against Human PINK1 were performed. The affinity constant is 125 nM.

PINK1 Recombinant monoclonal antibody, PBS Only

PINK1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 81991-4-PBS

产品说明书

CloneNo. 240449E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Indirect ELISA

别名

240449E7, BRPK, EC:2.7.11.1, PARK6, PTEN induced putative kinase 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PINK1 Ready-To-Use IHC Kit. PINK1 primary antibody included.

产品信息

81991-4-PBS targets PINK1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19825

Product name: Recombinant human PINK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 336-581 aa of BC028215

Sequence: PRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PTEN induced putative kinase 1
别名 240449E7, BRPK, EC:2.7.11.1, PARK6, PTEN induced putative kinase 1
计算分子量 581 aa, 63 kDa
GenBank蛋白编号BC028215
基因名称 PINK1
Gene ID (NCBI) 65018
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BXM7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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