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验证数据展示

  • WB analysis using 81991-4-RR (same clone as 81991-4-PBS)

    WB analysis using 81991-4-RR (same clone as 81991-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81991-4-RR (PINK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81991-4-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 81991-4-RR (same clone as 81991-4-PBS)

    FC experiment of Jurkat using 81991-4-RR (same clone as 81991-4-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PINK1 Recombinant antibody (81991-4-RR, Clone:240449E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81991-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00404-3

    Cytometric bead array standard curve of MP00404-3

    Cytometric bead array standard curve of MP00404-3, PINK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81991-4-PBS. Detection antibody: 81991-2-PBS. Standard: Ag19825. Range: 1.56-100 ng/mL.

  • Affinity and Kinetic Characterization of 81991-4-RR

    Affinity and Kinetic Characterization of 81991-4-RR

    Biolayer interferometry (BLl) kinetic assays of 81991-4-RR against Human PINK1 were performed. The affinity constant is 125 nM.

PINK1 Recombinant antibody, PBS Only (Capture)

PINK1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 81991-4-PBS

产品说明书

CloneNo. 240449E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

PTEN-induced putative kinase protein 1, PTEN induced putative kinase 1, PARK6, EC:2.7.11.1, BRPK

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PINK1 Ready-To-Use IHC Kit. PINK1 primary antibody included.

产品信息

81991-4-PBS targets PINK1 as part of a matched antibody pair:

MP00404-3: 81991-4-PBS capture and 81991-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PINK1 fusion protein Ag19825 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PTEN induced putative kinase 1
别名 PTEN-induced putative kinase protein 1, PTEN induced putative kinase 1, PARK6, EC:2.7.11.1, BRPK
计算分子量 581 aa, 63 kDa
GenBank蛋白编号BC028215
基因名称 PINK1
Gene ID (NCBI) 65018
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BXM7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C