验证数据展示
经过测试的应用
| Positive WB detected in | 3T3-L1 cells, LNCaP cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
27196-1-AP targets PIM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag25743 Product name: Recombinant human PIM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 244-313 aa of BC020224 Sequence: HDEEIIRGQVFFRQRVSSECQHLIRWCLALRPSDRPTFEEIQNHPWMQDVLLPQETAEIHLHSLSPGPSK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pim-1 oncogene |
| 别名 | PIM, pim 1 oncogene |
| 计算分子量 | 45 kDa |
| 观测分子量 | 30-40 kDa |
| GenBank蛋白编号 | BC020224 |
| 基因名称 | PIM1 |
| Gene ID (NCBI) | 5292 |
| RRID | AB_3742218 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P11309 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIM1 belongs to the Ser/Thr protein kinase family and the Pim subfamily. This family includes PIM1, PIM2, and PIM3, which share high sequence and structural similarity. PIM1 is a serine/threonine protein kinase that significantly promotes cell survival and proliferation, providing a selective advantage for tumorigenesis. PIM1 is expressed primarily in cells of the hematopoietic and germline lineages (PMID: 16186805).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PIM1 antibody 27196-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem The ubiquitin ligase Triad1 influences myeloid leukemogenesis by regulating the integrated stress response. | ||
PLoS Comput Biol An integrative analysis reveals the mechanism of plastic stabilizers inducing breast cancer. | ||
World J Surg Oncol Role and mechanism of PIM family in the immune microenvironment of diffuse large B cell lymphoma | ||
Phytomedicine Wogonin ameliorates osteoarthritis by targeting the STAT3/PIM1 axis to attenuate chondrocyte senescence. |
