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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 27196-1-AP

    WB analysis using 27196-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27196-1-AP (PIM1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

PIM1 Polyclonal antibody

PIM1 Polyclonal Antibody for WB, ELISA
Cat No. 27196-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

PIM, pim 1 oncogene

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected in3T3-L1 cells, LNCaP cells
Planning an IHC experiment? We recommend our IHCeasy PIM1 Ready-To-Use IHC Kit. PIM1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27196-1-AP targets PIM1 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 PIM1 fusion protein Ag25743 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pim-1 oncogene
别名 PIM, pim 1 oncogene
计算分子量 45 kDa
观测分子量 30-40 kDa
GenBank蛋白编号BC020224
基因名称 PIM1
Gene ID (NCBI) 5292
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11309
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIM1 belongs to the Ser/Thr protein kinase family and the Pim subfamily. This family includes PIM1, PIM2, and PIM3, which share high sequence and structural similarity. PIM1 is a serine/threonine protein kinase that significantly promotes cell survival and proliferation, providing a selective advantage for tumorigenesis. PIM1 is expressed primarily in cells of the hematopoietic and germline lineages (PMID: 16186805).

实验方案

Product Specific Protocols
WB protocol for PIM1 antibody 27196-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols