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验证数据展示

  • WB analysis using 11818-1-AP

    WB analysis using 11818-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11818-1-AP (PILRA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PILRA Polyclonal antibody

PILRA Polyclonal Antibody for WB, ELISA
Cat No. 11818-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Cell surface receptor FDF03, FDF03, Inhibitory receptor PILR alpha, PILRA, PILRα

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11818-1-AP targets PILRA in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 PILRA fusion protein Ag2385 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 paired immunoglobin-like type 2 receptor alpha
别名 Cell surface receptor FDF03, FDF03, Inhibitory receptor PILR alpha, PILRA, PILRα
计算分子量 303 aa, 34 kDa
观测分子量50 kDa
GenBank蛋白编号BC017812
基因名称 PILRA
Gene ID (NCBI) 29992
RRIDAB_2163648
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKJ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Paired receptors consist of highly related activating and inhibitory receptors and are widely involved in the regulation of the immune system. Paired immunoglobulin-like type 2 receptor alpha (PILRα) and PILRβ are paired receptors bearing highly similar extracellular ligand-binding domains but divergent intracellular signaling domains. PILRα is the immune inhibitory receptor possessing an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain enabling it to deliver inhibitory signals. Binding of PILRα to its ligand CD99 is involved in immune regulation. PILRα can also recognize other ligands, PILR-associating neural protein (PANP), and HSV-1 glycoprotein B (HSV-1 gB). PILRα has a calculated molecular weight of 34 kDa. It has been reported that PILRα migrates at 44-50 kDa due to glycosylation (PMID: 10903717; 10660620). The other band of about 61 kDa detected by this antibody may present a heavily glycosylated form of PILRα.

实验方案

Product Specific Protocols
WB protocol for PILRA antibody 11818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols