验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HUVEC cells, HEK-293 cells |
| Positive IHC detected in | human kidney tissue, mouse testis tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 3 publications below |
产品信息
27035-1-AP targets PI3 Kinase p55 Gamma in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag25884 Product name: Recombinant human PIK3R3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 312-382 aa of BC021622 Sequence: HLVWLNHKGVRQKRLNVWLGIKNEDAAENYFINEEDENLPHYDEKTWFVEDINRVQAEDLLYGKPDGAFLI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphoinositide-3-kinase, regulatory subunit 3 (gamma) |
| 别名 | p55, p55 GAMMA, p55PIK, PI3 Kinase p55 Gamma, PI3 kinase subunit p55 gamma, PI3K regulatory subunit gamma, PIK3R3 |
| 计算分子量 | 85 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC021622 |
| 基因名称 | PI3 Kinase p55 Gamma |
| Gene ID (NCBI) | 8503 |
| RRID | AB_2880730 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92569 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIK3R3, also named as p55PIK, belongs to the PI3K p85 subunit family. It binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During INS stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The antibody is specific to PIK3R3.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PI3 Kinase p55 Gamma antibody 27035-1-AP | Download protocol |
| IHC protocol for PI3 Kinase p55 Gamma antibody 27035-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Physiol Lung Cell Mol Physiol p55PIK Deficiency and its N-terminal Derivative inhibit Inflammation and Emphysema in COPD mouse model. | ||
BMC Cancer Circ_0021350 plays an oncogene role by regulating miR-1207-3p/PIK3R3 in glioblastoma
| ||
World J Mens Health Relaxin-2 Prevents Erectile Dysfunction by Cavernous Nerve, Endothelial and Histopathological Protection Effects in Rats with Bilateral Cavernous Nerve Injury | ||
Mol Oncol Consensus molecular subtyping of colorectal carcinoma brain metastases reveals a metabolic signature associated with poor patient survival |