验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, HepG2 cells, mouse liver tissue, PC-3 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
18334-1-AP targets PIGS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag12950 Product name: Recombinant human PIGS protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 313-555 aa of BC001319 Sequence: SSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIMVYNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFLETRKSWRKPEKTD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphatidylinositol glycan anchor biosynthesis, class S |
| 别名 | UNQ1873/PRO4316, Phosphatidylinositol-glycan biosynthesis class S protein, GPI transamidase component PIG-S |
| 计算分子量 | 62 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC001319 |
| 基因名称 | PIGS |
| Gene ID (NCBI) | 94005 |
| RRID | AB_2878532 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q96S52 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIGS, also named as GPI transamidase component PIG-S or PRO4316, is a 555 amino acid protein, which belongs to the PIGS family. PIGS localizes in the endoplasmic reticulum membrane. PIGS is a component of the GPI transamidase complex. It is essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PIGS antibody 18334-1-AP | Download protocol |
| WB protocol for PIGS antibody 18334-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2. | ||
Nat Commun Proteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its role in glycosylphosphatidylinositol-anchored protein biogenesis |
