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验证数据展示

  • WB analysis of HeLa using 18334-1-AP

    WB analysis of HeLa using 18334-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18334-1-AP (PIGS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 18334-1-AP

    IF Staining of HepG2 using 18334-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PIGS antibody (18334-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PIGS Polyclonal antibody

PIGS Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 18334-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

UNQ1873/PRO4316, Phosphatidylinositol-glycan biosynthesis class S protein, GPI transamidase component PIG-S

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HepG2 cells, mouse liver tissue, PC-3 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

18334-1-AP targets PIGS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 PIGS fusion protein Ag12950 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatidylinositol glycan anchor biosynthesis, class S
别名 UNQ1873/PRO4316, Phosphatidylinositol-glycan biosynthesis class S protein, GPI transamidase component PIG-S
计算分子量 62 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC001319
基因名称 PIGS
Gene ID (NCBI) 94005
RRIDAB_2878532
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ96S52
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIGS, also named as GPI transamidase component PIG-S or PRO4316, is a 555 amino acid protein, which belongs to the PIGS family. PIGS localizes in the endoplasmic reticulum membrane. PIGS is a component of the GPI transamidase complex. It is essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates.

实验方案

Product Specific Protocols
WB protocol for PIGS antibody 18334-1-APDownload protocol
IF protocol for PIGS antibody 18334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Identification of mammalian glycoproteins with type-I LacdiNAc structures synthesized by the glycosyltransferase B3GALNT2.

Authors - Takahiro Nakane
View Article
human,mouseWB

Nat Commun

Proteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its role in glycosylphosphatidylinositol-anchored protein biogenesis

Authors - Xiaoqiong Wei
View Article