验证数据展示
经过测试的应用
| Positive WB detected in | human liver tissue, human brain tissue, mouse testis tissue, Raji cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
12612-1-AP targets PIGB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag3244 Product name: Recombinant human PIGB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-335 aa of BC017711 Sequence: MRRPLSKCGMEPGGGDASLTLHGLQNRSHGKIKLRKRKSTLYFNTQEKSARRRGDLLGENIYLLLFTLALRILNCFLVQTSFVPDEYWQSLEVSHHMVFNYGYLTWEWTERLRSYTYPLIFASIYKILHLLGKDSVQLLIWIPRLAQALLSAVADVRLYSLMKQLENQEVARWVFFCQLCSWFTWYCCTRTLTNTMETVLTIIALFYYPLEGSKSMNSVKYSSLVALAFIIRPTAVILWTPLLFRHFCQEPRKLDLILHHFLPVGFVTLSLSLMIDRIFFGQWTLVQFNFLKFNVLQNLGTFYGSHPWHWYFSQGFPVILGTHLPFFIHGCYLAP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphatidylinositol glycan anchor biosynthesis, class B |
| 别名 | GPI mannosyltransferase 3, GPI mannosyltransferase III, GPI MT III, PIG B, PIGB |
| 计算分子量 | 65 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC017711 |
| 基因名称 | PIGB |
| Gene ID (NCBI) | 9488 |
| RRID | AB_2164664 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92521 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PIGB antibody 12612-1-AP | Download protocol |
| WB protocol for PIGB antibody 12612-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem J Accelerated urokinase-receptor protein turnover triggered by interference with the addition of the glycolipid anchor. | ||
Biomed Res Int Cell Cycle-Related Gene SPC24: A Novel Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Cancer |
