验证数据展示
经过测试的应用
| Positive WB detected in | rat liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33859-1-AP targets PID1 in WB, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag40871 Product name: Recombinant human PID1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-168 aa of BC040164 Sequence: MWQPATERLQVTYLGKVSTTGMQFLSGCTEKPVIELWKKHTLAREDVFPANALLEIRPFQVWLHHLDHKGEATVHMDTFQVARIAYCTADHNVSPNIFAWVYREINDDLSYQMDCHAVECESKLEAKKLAHAMMEAFRKTFHSMKSDGRIHSNSSSEEVSQELESDDG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphotyrosine interaction domain containing 1 |
| 别名 | |
| 计算分子量 | 168 aa, 19 kDa |
| 观测分子量 | 25 kDa, 28 kDa |
| GenBank蛋白编号 | BC040164 |
| 基因名称 | PID1 |
| Gene ID (NCBI) | 55022 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q7Z2X4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PID1 is a cytoplasmic PTB-domain adaptor that negatively regulates insulin and IGF1 signaling, modulating AKT activity, glucose uptake, and mitochondrial function. Enriched in muscle, adipose tissue, and liver, PID1 promotes insulin resistance, impacts lipid handling, and contributes to metabolic disease. Its dysregulation also influences tumor metabolism and stress responses, underscoring PID1 as an important node linking growth factor signaling, energy homeostasis, and pathophysiology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PID1 antibody 33859-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
