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PICALM Monoclonal antibody, PBS Only

PICALM Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

1F6G7

Cat No : 67564-1-PBS

Print datasheet

Synonyms

CALM, CLTH, LAP, PICALM

验证数据展示

  • WB analysis using 67564-1-Ig (same clone as 67564-1-PBS)

    WB analysis using 67564-1-Ig (same clone as 67564-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67564-1-Ig (PICALM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67564-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67564-1-Ig (same clone as 67564-1-PBS)

    IHC staining of human lung cancer using 67564-1-Ig (same clone as 67564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67564-1-Ig (PICALM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67564-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67564-1-Ig (same clone as 67564-1-PBS)

    IF Staining of HeLa using 67564-1-Ig (same clone as 67564-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PICALM antibody (67564-1-Ig, Clone: 1F6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67564-1-PBS in a different storage buffer formulation.

产品信息

67564-1-PBS targets PICALM in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,IHC,IF,ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen PICALM fusion protein Ag29512 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphatidylinositol binding clathrin assembly protein
Synonyms CALM, CLTH, LAP, PICALM
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC064357
Gene Symbol PICALM
Gene ID (NCBI) 8301
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13492
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS). (PMID: 33397371, PMID: 26075887, PMID: 26005850)