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验证数据展示

  • WB analysis using 28554-1-AP

    WB analysis using 28554-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28554-1-AP (PICALM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28554-1-AP

    IHC staining of mouse brain using 28554-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28554-1-AP (PICALM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PICALM antibody included

PICALM Polyclonal antibody

PICALM Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28554-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

CALM, CLTH, LAP, PICALM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, K-562 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PICALM Ready-To-Use IHC Kit. PICALM primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28554-1-AP targets PICALM in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PICALM fusion protein Ag29505 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatidylinositol binding clathrin assembly protein
别名 CALM, CLTH, LAP, PICALM
计算分子量 71 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC064357
基因名称 PICALM
Gene ID (NCBI) 8301
RRIDAB_2918175
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13492
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PICALM (Phosphatidyl Inositol Clathrin Assembly Lymphoid Myeloid protein) is a ubiquitously expressed protein that plays a role in clathrin-mediated endocytosis. PICALM also affects the internalization and trafficking of SNAREs and modulates macroautophagy. PICALM is a highly validated genetic risk factor for Alzheimer's disease (AD). The long isoform of human PICALM has 22 exons (encoding PICALML), while the short isoform lacks exon 14 (encoding PICALMS). (PMID: 33397371, PMID: 26075887, PMID: 26005850)

实验方案

Product Specific Protocols
WB protocol for PICALM antibody 28554-1-APDownload protocol
IHC protocol for PICALM antibody 28554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols