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验证数据展示

  • WB analysis using 14413-1-AP (same clone as 14413-1-PBS)

    WB analysis using 14413-1-AP (same clone as 14413-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14413-1-AP (PIBF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 14413-1-AP (same clone as 14413-1-PBS)

    IP experiment of HEK-293 using 14413-1-AP (same clone as 14413-1-PBS)

    IP result of anti-PIBF1 (IP:14413-1-AP, 4ug; Detection:14413-1-AP 1:1000) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 14413-1-AP (same clone as 14413-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 14413-1-AP (same clone as 14413-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14413-1-AP (PIBF1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PIBF1 antibody included
  • IF Staining of HEK-293 using 14413-1-AP (same clone as 14413-1-PBS)

    IF Staining of HEK-293 using 14413-1-AP (same clone as 14413-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using PIBF1 antibody (14413-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8, red). This data was developed using the same antibody clone with 14413-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PIBF1 Polyclonal antibody, PBS Only

PIBF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14413-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PIBF1 Ready-To-Use IHC Kit. PIBF1 primary antibody included.

产品信息

14413-1-PBS targets PIBF1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5755

Product name: Recombinant human PIBF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 452-757 aa of BC051911

Sequence: KVTEFLHQSKLKSFESERVQLLQEETARNLTQCQLECEKYQKKLEVLTKEFYSLQASSEKRITELQAQNSEHQARLDIYEKLEKELDEIIMQTAEIENEDEAERVLFSYGYGANVPTTAKRRLKQSVHLARRVLQLEKQNSLILKDLEHRKDQVTQLSQELDRANSLLNQTQQPYRYLIESVRQRDSKIDSLTESIAQLEKDVSNLNKEKSALLQTKNQMALDLEQLLNHREELAAMKQILVKMHSKHSENSLLLTKTEPKHVTENQKSKTLNVPKEHEDNIFTPKPTLFTKKEAPEWSKKQKMKT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 progesterone immunomodulatory binding factor 1
别名
计算分子量 90 kDa
观测分子量 90 kDa
GenBank蛋白编号BC051911
基因名称 PIBF1
Gene ID (NCBI) 10464
RRIDAB_10858916
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WXW3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PIBF1 is induced by the steroid hormone progesterone and plays a role in the maintenance of pregnancy. PIBF1 regulates multiple facets of the immune system to promote normal pregnancy including cytokine synthesis, natural killer (NK) cell activity, and arachidonic acid metabolism. Low serum levels of this protein have been associated with spontaneous pre-term labor in humans. PIBF1 may promote the proliferation, migration and invasion of glioma.

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