PI4KB Polyclonal antibody
PI4KB Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, NIH-3T3 cells (RNAi) |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13247-1-AP targets PI4KB in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, IP, WB, ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | PI4KB fusion protein Ag3989 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | phosphatidylinositol 4-kinase, catalytic, beta |
| Synonyms | PI4 kinase beta, PI4K BETA, pi4K92, PI4KB, PI4Kbeta, PI4KIIIbeta, PIK4CB, PtdIns 4 kinase beta |
| Calculated Molecular Weight | 801 aa, 90 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC040300 |
| Gene Symbol | PI4KB |
| Gene ID (NCBI) | 5298 |
| RRID | AB_2252370 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBF8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PI4KB antibody 13247-1-AP | Download protocol |
| IHC protocol for PI4KB antibody 13247-1-AP | Download protocol |
| IP protocol for PI4KB antibody 13247-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity | ||
EMBO J Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB. | ||
PLoS Pathog Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. | ||
Virol Sin Construction of coxsackievirus B5 viruses with luciferase reporters and their applications in vitro and in vivo | ||
Cereb Cortex Neonatal exposure to sevoflurane induces adolescent neurobehavioral dysfunction by interfering with hippocampal glycerophoslipid metabolism in rats. | ||
J Cell Sci Depletion of phosphatidylinositol 4-phosphate at the Golgi translocates K-Ras to mitochondria. |
