Various lysates were subjected to SDS PAGE followed by western blot with 82938-1-RR (PI4KA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 82938-1-RR (PI4KA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82938-1-RR (PI4KA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PI4KA antibody (82938-1-RR, Clone: 230057G2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PI4KA Recombinant antibody (82938-1-RR, Clone:230057G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82938-1-PBS in a different storage buffer formulation.

Biolayer interferometry (BLl) kinetic assays of 82938-1-RR against Human PI4KA were performed. The affinity constant is below 1 pM.