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验证数据展示

  • WB analysis using 67121-1-Ig (same clone as 67121-1-PBS)

    WB analysis using 67121-1-Ig (same clone as 67121-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67121-1-Ig (PIK3CB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67121-1-Ig (same clone as 67121-1-PBS)

    IHC staining of human lung cancer using 67121-1-Ig (same clone as 67121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67121-1-Ig (PIK3CB antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67121-1-Ig (same clone as 67121-1-PBS)

    IHC staining of human lung cancer using 67121-1-Ig (same clone as 67121-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67121-1-Ig (PIK3CB antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67121-1-Ig (same clone as 67121-1-PBS)

    IF Staining of HeLa using 67121-1-Ig (same clone as 67121-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PI3 Kinase p110 Beta antibody (67121-1-Ig, Clone: 1B2A1 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 67121-1-PBS in a different storage buffer formulation.

PI3 Kinase p110 Beta Monoclonal antibody, PBS Only

PI3 Kinase p110 Beta Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67121-1-PBS

产品说明书

CloneNo. 1B2A1

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PI3K p110(beta), PIK3CB, PI3K, PI3 kinase subunit beta, p110 BETA

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67121-1-PBS targets PI3 Kinase p110 Beta in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag17505

Product name: Recombinant human PIK3CB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 139-373 aa of BC114432

Sequence: SLKDPEVNEFRRKMRKFSEEKILSLVGLSWMDWLKQTYPPEHEPSIPENLEDKLYGGKLIVAVHFENCQDVFSFQVSPNMNPIKVNELAIQKRLTIHGKEDEVSPYDYVLQVSGRVEYVFGDHPLIQFQYIRNCVMNRALPHFILVECCKIKKMYEQEMIAIEAAINRNSSNLPLPLPPKKTRIISHVWENNNPFQIVLVKGNKLNTEETVKVHVRAGLFHGTELLCKTIVSSEV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoinositide-3-kinase, catalytic, beta polypeptide
别名 PI3K p110(beta), PIK3CB, PI3K, PI3 kinase subunit beta, p110 BETA
计算分子量 1070 aa, 123 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC114432
基因名称 PI3 Kinase p110 Beta
Gene ID (NCBI) 5291
RRIDAB_2882424
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP42338
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002).

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