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验证数据展示

  • WB analysis using 82796-4-RR

    WB analysis using 82796-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82796-4-RR (PIK3CA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82796-4-RR

    Affinity and Kinetic Characterization of 82796-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82796-4-RR against Human PI3 Kinase p110 Alpha were performed. The affinity constant is 0.639 nM.

PI3 Kinase p110 Alpha Recombinant antibody

PI3 Kinase p110 Alpha Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 82796-4-RR

产品说明书

CloneNo. 1B4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

PI3K p110(alpha), PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, p110 alpha

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, COLO 320 cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Planning an IHC experiment? We recommend our IHCeasy PIK3CA Ready-To-Use IHC Kit. PIK3CA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82796-4-RR targets PI3 Kinase p110 Alpha in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PI3 Kinase p110 Alpha fusion protein Ag16586 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphoinositide-3-kinase, catalytic, alpha polypeptide
别名 PI3K p110(alpha), PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, p110 alpha
计算分子量 1068 aa, 124 kDa
观测分子量110 kDa
GenBank蛋白编号BC113601
基因名称 PIK3CA
Gene ID (NCBI) 5290
RRIDAB_3086541
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP42336
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

实验方案

Product Specific Protocols
WB protocol for PI3 Kinase p110 Alpha antibody 82796-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols