验证数据展示
经过测试的应用
| Positive IHC detected in | human skin cancer tissue, human oesophagus tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 4 publications below |
产品信息
15963-1-AP targets Elafin/Skalp in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, pig |
| 免疫原 |
CatNo: Ag8735 Product name: Recombinant human PI3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-117 aa of BC010952 Sequence: AAVTGVPVKGQDTVKGRVPFNGQDPVKGQVSVKGQDKVKAQEPVKGPVSTKPGSCPIILIRCAMLNPPNRCLKDTDCPGIKKCCEGSCGMACFVPQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | peptidase inhibitor 3, skin-derived |
| 别名 | cementoin, Elafin, Elastase specific inhibitor, ESI, Peptidase inhibitor 3, PI 3, PI3, Protease inhibitor WAP3, SKALP, WAP3, WFDC14 |
| 计算分子量 | 117 aa, 12 kDa |
| GenBank蛋白编号 | BC010952 |
| 基因名称 | Elafin/Skalp |
| Gene ID (NCBI) | 5266 |
| RRID | AB_2878201 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P19957 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Elafin/Skalp antibody 15963-1-AP | Download protocol |
| IHC protocol for Elafin/Skalp antibody 15963-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Ther Adv Med Oncol Development and external validation of a composite immune-clinical prognostic model associated with EGFR mutation in East-Asian patients with lung adenocarcinoma. | ||
Anim Biosci Spatiotemporal expression and regulation of peptidase inhibitor 3 and secretory leukocyte protease inhibitor at the maternal-fetal interface in pigs | ||
Int J Gen Med FAM3B Serves as a Biomarker for the Development and Malignancy of Oral Lichen Planus. | ||
Front Endocrinol (Lausanne) Elafin is related to immune infiltration and could predict the poor prognosis in ovarian cancer |
