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验证数据展示

  • WB analysis of mouse brain using 16984-1-AP

    WB analysis of mouse brain using 16984-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16984-1-AP (PHYHIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PHYHIP Polyclonal antibody

PHYHIP Polyclonal Antibody for WB, ELISA
Cat No. 16984-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

DYRK1AP3, KIAA0273, PAHX AP, PAHX AP1, PAHXAP1, PHYHIP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16984-1-AP targets PHYHIP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10575

Product name: Recombinant human PHYHIP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-330 aa of BC034034

Sequence: MELLSTPHSIEINNITCDSFSISWAMEDSDLERVTHYFIDLNKKENKNSNKFKHRDVPTKLVAKAVPLPMTVRGHWFLSPRTEYSVAVQTAVKQSDGEYLVSGWSETVEFCTGDYAKEHLAQLQEKAEQIAGRMLRFSVFYRNHHKEYFEHARTHCGNMLQPYLKDNSGSHGSPTSGMLHGVFFSCNTEFNTGQPPQDSPYGRWRFQIPAQRLFNPSTNLYFADFYCMYTAYHYAILVLAPKGSMGDRFCRDRRPLLDIACNKFLTCSVEDGELVFRHAQDLILEIIYTEPVDLSLGTLGEISGHQLMSLSTADAKKDPSCKTCNISVGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phytanoyl-CoA 2-hydroxylase interacting protein
别名 DYRK1AP3, KIAA0273, PAHX AP, PAHX AP1, PAHXAP1, PHYHIP
计算分子量 330 aa, 38 kDa
观测分子量 35 kDa, 43-45 kDa
GenBank蛋白编号BC034034
基因名称 PHYHIP
Gene ID (NCBI) 9796
RRIDAB_2163759
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92561
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PHYHIP, also named as DYRK1AP3 and KIAA0273, belongs to the PHYHIP family. Its interaction with PHYH suggests a role in the development of the central system. This antibody recognizes both PHYHIP (35-38 kDa) and PHYHIPL (43-45 kDa).

实验方案

Product Specific Protocols
WB protocol for PHYHIP antibody 16984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols