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验证数据展示

  • WB analysis of MCF-7 using 13112-1-AP

    WB analysis of MCF-7 using 13112-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13112-1-AP (PHPT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 13112-1-AP

    IHC staining of mouse heart using 13112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13112-1-AP (PHPT1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 13112-1-AP

    IF Staining of HeLa using 13112-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PHPT1 antibody (13112-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PHPT1 Polyclonal antibody

PHPT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13112-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CGI-202, EC:3.9.1.3, HSPC141, PHP, PHP14

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF7 cells, human kidney tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13112-1-AP targets PHPT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3778

Product name: Recombinant human PHPT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-125 aa of BC024648

Sequence: MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDGY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphohistidine phosphatase 1
别名 CGI-202, EC:3.9.1.3, HSPC141, PHP, PHP14
计算分子量 125 aa, 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC024648
基因名称 PHPT1
Gene ID (NCBI) 29085
RRIDAB_2163737
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRX4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for PHPT1 antibody 13112-1-APDownload protocol
IHC protocol for PHPT1 antibody 13112-1-APDownload protocol
WB protocol for PHPT1 antibody 13112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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