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验证数据展示

  • WB analysis of Neuro-2a using 66254-1-Ig (same clone as 66254-1-PBS)

    WB analysis of Neuro-2a using 66254-1-Ig (same clone as 66254-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66254-1-Ig (PHOX2B Antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

  • IHC staining of neuroblastoma using 66254-1-Ig (same clone as 66254-1-PBS)

    IHC staining of neuroblastoma using 66254-1-Ig (same clone as 66254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 66254-1-Ig (PHOX2B antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 66254-1-Ig (same clone as 66254-1-PBS)

    IF Staining of Neuro-2a using 66254-1-Ig (same clone as 66254-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PHOX2B antibody (66254-1-Ig, Clone: 1F5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 66254-1-Ig (same clone as 66254-1-PBS)

    IF Staining of SH-SY5Y using 66254-1-Ig (same clone as 66254-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PHOX2B antibody (66254-1-Ig, Clone: 1F5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66254-1-PBS in a different storage buffer formulation.

PHOX2B Monoclonal antibody, PBS Only

PHOX2B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66254-1-PBS

产品说明书

CloneNo. 1F5F5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

NBPhox, Neuroblastoma Phox, paired like homeobox 2b, PHOX2B, PHOX2B homeodomain protein, PMX2B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66254-1-PBS targets PHOX2B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13196

Product name: Recombinant human PHOX2B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 63-314 aa of BC017199

Sequence: GSCSLGTLRDHQSSPYAAVPYKLFTDHGGLNEKRKQRRIRTTFTSAQLKELERVFAETHYPDIYTREELALKIDLTEARVQVWFQNRRAKFRKQERAAAAAAAAAKNGSSGKKSDSSRDDESKEAKSTDPDSTGGPGPNPNPTPSCGANGGGGGGPSPAGAPGAAGPGGPGGEPGKGGAAAAAAAAAAAAAAAAAAAAGGLAAAGGPGQGWAPGPGPITSIPDSLGGPFASVLSSLQRPNGAKAALVKSSMF

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 paired-like homeobox 2b
别名 NBPhox, Neuroblastoma Phox, paired like homeobox 2b, PHOX2B, PHOX2B homeodomain protein, PMX2B
计算分子量 31 kDa
观测分子量 32-36 kDa
GenBank蛋白编号BC017199
基因名称 PHOX2B
Gene ID (NCBI) 8929
RRIDAB_2881642
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ99453
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.

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