PHOX2B Polyclonal antibody
PHOX2B Polyclonal Antibody for IF/ICC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
IF/ICC, IHC, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Neuro-2a cells, SH-SY5Y cells |
| Positive IHC detected in | neuroblastoma tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Neuro-2a cells, SH-SY5Y cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 25276-1-AP is PHOX2B Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC, IHC, WB,ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | PHOX2B fusion protein Ag13196 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | paired-like homeobox 2b |
| Synonyms | NBPhox, Neuroblastoma Phox, paired like homeobox 2b, PHOX2B, PHOX2B homeodomain protein, PMX2B |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC017199 |
| Gene Symbol | PHOX2B |
| Gene ID (NCBI) | 8929 |
| RRID | AB_2880001 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99453 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PHOX2B antibody 25276-1-AP | Download protocol |
| IHC protocol for PHOX2B antibody 25276-1-AP | Download protocol |
| IF protocol for PHOX2B antibody 25276-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Stem Cells Transl Med Neural Stem Cell-Derived Exosomal Netrin1 Contributes to Neuron Differentiation of Mesenchymal Stem Cells in Therapy of Spinal Bifida Aperta. | ||
Mol Metab Ablation of intact hypothalamic and/or hindbrain TrkB signaling leads to perturbations in energy balance. | ||
Sci Rep Selective Induction of Human Autonomic Neurons Enables Precise Control of Cardiomyocyte Beating. | ||
Int J Mol Sci Deciphering the Molecular Mechanisms of Autonomic Nervous System Neuron Induction through Integrative Bioinformatics Analysis |
