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验证数据展示

  • WB analysis using 11869-1-AP (same clone as 11869-1-PBS)

    WB analysis using 11869-1-AP (same clone as 11869-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11869-1-AP (PHOSPHO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11869-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 11869-1-AP (same clone as 11869-1-PBS)

    IHC staining of mouse testis using 11869-1-AP (same clone as 11869-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11869-1-AP (PHOSPHO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11869-1-PBS in a different storage buffer formulation.

PHOSPHO2 Polyclonal antibody, PBS Only

PHOSPHO2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11869-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, Indirect ELISA

别名

EC:3.1.3.74

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11869-1-PBS targets PHOSPHO2 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Ag2451

Product name: Recombinant human PHOSPHO2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-241 aa of BC022324

Sequence: MKILLVFDFDNTIIDDNSDTWIVQCAPNKKLPIELRDSYRKGFWTEFMGRVFKYLGDKGVREHEMKRAVTSLPFTPGMVELFNFIRKNKDKFDCIIISDSNSVFIDWVLEAASFHDIFDKVFTNPAAFNSNGHLTVENYHTHSCNRCPKNLCKKVVLIEFVDKQLQQGVNYTQIVYIGDGGNDVCPVTFLKNDDVAMPRKGYTLQKTLSRMSQNLEPMEYSVVVWSSGVDIISHLQFLIKD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatase, orphan 2
别名 EC:3.1.3.74
计算分子量 241 aa, 28 kDa
观测分子量28 kDa
GenBank蛋白编号BC022324
基因名称 PHOSPHO2
Gene ID (NCBI) 493911
RRIDAB_3085383
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TCD6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHOSPHO2 belongs to the HAD-like hydrolase superfamily and PHOSPHO family. PHOSPHO2 is a phosphatase that has high activity toward pyridoxal 5'-phosphate (PLP). PHOSPHO2 is also active at much lower level toward pyrophosphate, phosphoethanolamine (PEA), phosphocholine (PCho), phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, and h-glycerophosphate. The molecular weight of PHOSPHO2 is 28 kDa.

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