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验证数据展示

  • WB analysis using 33225-1-AP (same clone as 33225-1-PBS)

    WB analysis using 33225-1-AP (same clone as 33225-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33225-1-AP (PHLDB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33225-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 33225-1-AP (same clone as 33225-1-PBS)

    IHC staining of human ovary tumor using 33225-1-AP (same clone as 33225-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 33225-1-AP (PHLDB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33225-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 33225-1-AP (same clone as 33225-1-PBS)

    IHC staining of human ovary tumor using 33225-1-AP (same clone as 33225-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 33225-1-AP (PHLDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33225-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 33225-1-AP (same clone as 33225-1-PBS)

    IHC staining of mouse heart using 33225-1-AP (same clone as 33225-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 33225-1-AP (PHLDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33225-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 33225-1-AP (same clone as 33225-1-PBS)

    IF Staining of U2OS using 33225-1-AP (same clone as 33225-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PHLDB1 antibody (33225-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 33225-1-PBS in a different storage buffer formulation.

PHLDB1 Polyclonal antibody, PBS Only

PHLDB1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 33225-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DLNB07, KIAA0638, LL5A, Pleckstrin Homology Like Domain Family B Member 1, Pleckstrin homology-like domain family B member 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

33225-1-PBS targets PHLDB1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag37254

Product name: Recombinant human PHLDB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 620-800 aa of BC098586

Sequence: LCAEYSRADGGPEAGELPSIGEATAALALAGRRPSRGLAGASGRSSEEPGVATQRLWESMERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGHVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVALETGIQKERDKEAEALET

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pleckstrin homology-like domain, family B, member 1
别名 DLNB07, KIAA0638, LL5A, Pleckstrin Homology Like Domain Family B Member 1, Pleckstrin homology-like domain family B member 1
观测分子量151 kDa
GenBank蛋白编号BC098586
基因名称 PHLDB1
Gene ID (NCBI) 23187
RRIDAB_3742903
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ86UU1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHLDB1 (Pleckstrin Homology-like Domain Family B Member 1), also known as LL5α, is a scaffolding protein that specifically binds to phosphatidylinositol-3,4,5-trisphosphate (PIP3) through its C-terminal PH domain, anchoring microtubule plus ends to the cell cortex. Functionally, PHLDB1 acts as an insulin-responsive protein that translocates from the cytoplasm to the plasma membrane upon insulin stimulation, promoting GLUT4 translocation and glucose uptake by enhancing Akt phosphorylation. In addition, PHLDB1 plays a key role in cell migration. Studies have shown that its genetic polymorphisms are associated with glioma risk, and by enhancing PI3K/AKT signaling, it promotes tumor cell invasion and metastasis, making it a potential prognostic marker and therapeutic target.

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