验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells, human placenta tissue, MCF-7 cells, PC-3 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
产品信息
14661-1-AP targets PHLDA2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, goat |
| 免疫原 |
CatNo: Ag6331 Product name: Recombinant human PHLDA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-152 aa of BC005034 Sequence: MKSPDEVLREGELEKRSDSLFQLWKKKRGVLTSDRLSLFPASPRARPKELRFHSILKVDCVERTGKYVYFTIVTTDHKEIDFRCAGESCWNAAIALALIDFQNRRALQDFRSRQERTAPAAPAEDAVAAAAAAPSEPSEPSRPSPQPKPRTP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pleckstrin homology-like domain, family A, member 2 |
| 别名 | BRW1C, BWR1C, HLDA2, IPL, p17 BWR1C, PHLDA2, TSSC3 |
| 计算分子量 | 17 kDa |
| 观测分子量 | 20-25 kDa |
| GenBank蛋白编号 | BC005034 |
| 基因名称 | PHLDA2 |
| Gene ID (NCBI) | 7262 |
| RRID | AB_2163294 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q53GA4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PHLDA2 antibody 14661-1-AP | Download protocol |
| WB protocol for PHLDA2 antibody 14661-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer. | ||
Aging (Albany NY) PHLDA2 regulates EMT and autophagy in colorectal cancer via the PI3K/AKT signaling pathway.
| ||
Pathol Res Pract FOSL1 transcriptionally regulates PHLDA2 to promote 5-FU resistance in colon cancer cells
| ||
Cell Metab PHLDA2-mediated phosphatidic acid peroxidation triggers a distinct ferroptotic response during tumor suppression |
