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PHKG2 Polyclonal antibody

PHKG2 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 15109-1-AP

Print datasheet

Synonyms

GSD9C, PHK gamma T, PHKG2, PHK-gamma-LT, Phosphorylase kinase subunit gamma-2, PSK C3

验证数据展示

  • IHC staining of human liver cancer using 15109-1-AP

    IHC staining of human liver cancer using 15109-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15109-1-AP (PHKG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 15109-1-AP

    IF Staining of HEK-293 using 15109-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PHKG2 antibody (15109-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of HEK-293 using 15109-1-AP

    WB analysis of HEK-293 using 15109-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 15109-1-AP

    IP experiment of HEK-293 using 15109-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15109-1-AP (PHKG2 antibody) at dilution of 1:300.

经过测试的应用

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15109-1-AP targets PHKG2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen PHKG2 fusion protein Ag6829 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphorylase kinase, gamma 2 (testis)
Synonyms GSD9C, PHK gamma T, PHKG2, PHK-gamma-LT, Phosphorylase kinase subunit gamma-2, PSK C3
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC002541
Gene Symbol PHKG2
Gene ID (NCBI) 5261
RRIDAB_2165529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15735
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PHKG2 antibody 15109-1-APDownload protocol
IHC protocol for PHKG2 antibody 15109-1-APDownload protocol
IF protocol for PHKG2 antibody 15109-1-APDownload protocol
IP protocol for PHKG2 antibody 15109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Sci Adv

Lactate metabolism is essential in early-onset mitochondrial myopathy

Authors - Zhenkang Chen
View Article