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PHKG1 Polyclonal antibody

PHKG1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 16743-1-AP

Print datasheet

Synonyms

PHKG, PHKG1

验证数据展示

  • WB analysis using 16743-1-AP

    WB analysis using 16743-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16743-1-AP (PHKG1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 16743-1-AP

    IHC staining of mouse skeletal muscle using 16743-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16743-1-AP (PHKG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 16743-1-AP

    IHC staining of mouse skeletal muscle using 16743-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16743-1-AP (PHKG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse skeletal muscle using 16743-1-AP

    IP experiment of mouse skeletal muscle using 16743-1-AP

    IP result of anti-PHKG1 (IP:16743-1-AP, 4ug; Detection:16743-1-AP 1:600) with mouse skeletal muscle tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16743-1-AP targets PHKG1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitypig
Immunogen PHKG1 fusion protein Ag10032 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphorylase kinase, gamma 1 (muscle)
Synonyms PHKG, PHKG1
Calculated Molecular Weight 387 aa, 45 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC051327
Gene Symbol PHKG1
Gene ID (NCBI) 5260
RRIDAB_1851532
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16816
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PHKG1(Phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform) is also named as phosphorylase kinase subunit gamma-1, PHKG and belongs to the CAMK Ser/Thr protein kinase family. Phosphorylase kinase is a crucial glycogenolytic regulatory enzyme. The holoenzymes are composed of 16 subunit proteins containing equimolar ratios of 4 subunit types known as alpha, beta, gamma, and delta. This protein is the skeletal muscle isoform of phosphorylase kinase and kinetic differences exist between the cardiac and adult skeletal muscle enzymes, and the liver and skeletal muscle enzymes are known to be separate isozymes.

实验方案

Product Specific Protocols
WB protocol for PHKG1 antibody 16743-1-APDownload protocol
IHC protocol for PHKG1 antibody 16743-1-APDownload protocol
IP protocol for PHKG1 antibody 16743-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

PLoS Genet

A Splice Mutation in the PHKG1 Gene Causes High Glycogen Content and Low Meat Quality in Pig Skeletal Muscle.

Authors - Junwu Ma
View Article