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PHKB Polyclonal antibody

PHKB Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13400-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IP, IHC, ELISA

PHKB, phosphorylase kinase, beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells, LNCaP cells, mouse heart tissue, rat heart tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse liver tissue, human skeletal muscle tissue, human liver tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13400-1-AP targets PHKB in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IP
经测试反应性 human, mouse
文献引用反应性human, mouse, pig
免疫原 PHKB fusion protein Ag4240 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphorylase kinase, beta
别名 PHKB, phosphorylase kinase, beta
计算分子量 1086 aa, 124 kDa
观测分子量 124 kDa
GenBank蛋白编号BC033657
基因名称 PHKB
Gene ID (NCBI) 5257
RRIDAB_2237183
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ93100
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PHKB gene encodes phosphorylase kinase subunit beta involved in glycan biosynthesis and glycogen metabolism. PHKB activity is regulated by phosphorylation of various serine residues, and catalyzes the phosphorylation of serine in certain substrates, including troponin I. Phosphorylase kinase (PhK) complex, composed of alpha, beta, gamma, and delta subunits, stimulates energy production from glycogen in the cascade activation of glycogenolysis. Its large homologous alpha and beta subunits regulate the activity of the catalytic gamma subunit. Defects in PHKB are the cause of glycogen storage disease type 9B (GSD9B) also known as phosphorylase kinase deficiency of liver and muscle (PKD), characterized by hepathomegaly, only slightly elevated transaminases and plasma lipids, clinical improvement with increasing age, and remarkably no clinical muscle involvement.

实验方案

Product Specific Protocols
WB protocol for PHKB antibody 13400-1-APDownload protocol
IHC protocol for PHKB antibody 13400-1-APDownload protocol
IP protocol for PHKB antibody 13400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIP, WB

Mol Endocrinol

20-HETE induces hyperglycemia through the cAMP/PKA-PhK-GP pathway.

Authors - Lai Guangrui G
mouseWB

iScience

Loss of RNA-Binding Protein Sfpq Causes Long-Gene Transcriptopathy in Skeletal Muscle and Severe Muscle Mass Reduction with Metabolic Myopathy.

Authors - Motoyasu Hosokawa
humanWB,IHC

J Cancer Res Clin Oncol

Phosphorylase kinase β affects colorectal cancer cell growth and represents a novel prognostic biomarker.

Authors - Guanghui Wang
  • KD Validated
pigWB

J Biol Chem

Circular RNA circMYLK4 shifts energy metabolism from glycolysis to OXPHOS by binding to the calcium channel auxiliary subunit CACNA2D2

Authors - Haigang Cao
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