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PHIP Polyclonal antibody

PHIP Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20933-1-AP

Print datasheet

Synonyms

ndrp, PH interacting protein, PHIP

验证数据展示

  • WB analysis using 20933-1-AP

    WB analysis using 20933-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20933-1-AP (PHIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of A375 using 20933-1-AP

    IP experiment of A375 using 20933-1-AP

    IP result of anti-PHIP (IP:20933-1-AP, 4ug; Detection:20933-1-AP 1:3000) with A375 cells lysate 3600ug.

  • IF Staining of MCF-7 using 20933-1-AP

    IF Staining of MCF-7 using 20933-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 20933-1-AP (PHIP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, A375 cells, Jurkat cells, MCF-7 cells
Positive IP detected inA375 cells
Positive IF detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20933-1-AP targets PHIP in WB, IP, IF, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB, IF, ChIP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen PHIP fusion protein Ag14968 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pleckstrin homology domain interacting protein
Synonyms ndrp, PH interacting protein, PHIP
Calculated Molecular Weight 1821 aa, 207 kDa
Observed Molecular Weight 207 kDa
GenBank Accession NumberBC008909
Gene Symbol PHIP
Gene ID (NCBI) 55023
RRIDAB_10733522
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWQ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PHIP is a protein that binds to the insulin receptor substrate 1 protein and regulates glucose transporter translocation in skeletal muscle cells. PHIP may also regulate growth and survival of pancreatic beta cells. Elevated copy number of this gene may be associated with melanoma severity and the encoded protein may promote melanoma metastasis in human patients.

实验方案

Product Specific Protocols
WB protocol for PHIP antibody 20933-1-APDownload protocol
IF protocol for PHIP antibody 20933-1-APDownload protocol
IP protocol for PHIP antibody 20933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Cell Metab

Exome Sequencing Identifies Genes and Gene Sets Contributing to Severe Childhood Obesity, Linking PHIP Variants to Repressed POMC Transcription.

Authors - Gaëlle Marenne
View Article
humanChIP

Cell Commun Signal

RepID represses megakaryocytic differentiation by recruiting CRL4A-JARID1A at DAB2 promoter

Authors - Jae-Hyun Jo
View Article
humanWB

BMC Cancer

LINC00955 suppresses colorectal cancer growth by acting as a molecular scaffold of TRIM25 and Sp1 to Inhibit DNMT3B-mediated methylation of the PHIP promoter

Authors - Ganglin Ren
View Article