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验证数据展示

  • WB analysis of HEK-293 using 81986-1-RR (same clone as 81986-1-PBS)

    WB analysis of HEK-293 using 81986-1-RR (same clone as 81986-1-PBS)

    WB result of PHGDH antibody (81986-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHGDH transfected HEK-293 cells. This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.

  • WB analysis using 81986-1-RR (same clone as 81986-1-PBS)

    WB analysis using 81986-1-RR (same clone as 81986-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81986-1-RR (PHGDH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 81986-1-RR (same clone as 81986-1-PBS)

    IP experiment of HeLa using 81986-1-RR (same clone as 81986-1-PBS)

    IP result of anti-PHGDH (IP:81986-1-RR, 4ug; Detection:81986-1-RR 1:10000) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 81986-1-RR (same clone as 81986-1-PBS)

    IHC staining of human colon cancer using 81986-1-RR (same clone as 81986-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81986-1-RR (PHGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81986-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PHGDH antibody included
  • Affinity and Kinetic Characterization of 81986-1-RR

    Affinity and Kinetic Characterization of 81986-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81986-1-RR against Human PHGDH were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

PHGDH Recombinant monoclonal antibody, PBS Only

PHGDH Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 81986-1-PBS

产品说明书

CloneNo. 5L16

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

EC:1.1.1.37, D-3-phosphoglycerate dehydrogenase, 5L16, 3-PGDH, 3PGDH

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PHGDH Ready-To-Use IHC Kit. PHGDH primary antibody included.

产品信息

81986-1-PBS targets PHGDH in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6445

Product name: Recombinant human PHGDH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 186-533 aa of BC000303

Sequence: FGVQQLPLEEIWPLCDFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSPKGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVNAKLLVKEAGLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNGAVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLVSDGETWHVMGISSLLPSLEAWKQHVTEAFQFHF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphoglycerate dehydrogenase
别名 EC:1.1.1.37, D-3-phosphoglycerate dehydrogenase, 5L16, 3-PGDH, 3PGDH
计算分子量 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC000303
基因名称 PHGDH
Gene ID (NCBI) 26227
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43175
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ).

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