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验证数据展示

  • WB analysis using 51062-1-AP

    WB analysis using 51062-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 51062-1-AP (PHF6 antibody) at dilution of 1:64000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 51062-1-AP

    IHC staining of human ovary tumor using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51062-1-AP (PHF6 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IHC staining of human placenta using 51062-1-AP

    IHC staining of human placenta using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IHC staining of human placenta using 51062-1-AP

    IHC staining of human placenta using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IHC staining of human stomach cancer using 51062-1-AP

    IHC staining of human stomach cancer using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IHC staining of mouse brain using 51062-1-AP

    IHC staining of mouse brain using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IHC staining of mouse stomach using 51062-1-AP

    IHC staining of mouse stomach using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IHC staining of rat stomach using 51062-1-AP

    IHC staining of rat stomach using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IF Staining of HeLa using 51062-1-AP

    IF Staining of HeLa using 51062-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PHF6 antibody (51062-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

PHF6 Polyclonal antibody

PHF6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 51062-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

BORJ, CENP-31, PHD-like zinc finger protein, PHF 6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman placenta tissue, human cervical cancer tissue, human ovary tumor tissue, human stomach cancer tissue, mouse brain tissue, mouse stomach tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PHF6 Ready-To-Use IHC Kit. PHF6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

51062-1-AP targets PHF6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag2102

Product name: Recombinant human PHF6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-312 aa of BC005994

Sequence: MSSSVEQKKGPTRQRKCGFCKSNRDKECGQLLISENQKVAAHHKCMLFSSALVSSHSDNESLGGFSIEDVQKEIKRGTKLMCSLCHCPGATIGCDVKTCHRTYHYHCALHDKAQIREKPSQGIYMAYCRKHKKTAHNSEAADLEESFNEHELEPSSPKSKKKSRKGRPRKTNFKGLSEDTRSTSSHGTDEMESSSYRDRSPHRSSPSDTRPKCGFCHVGEEENEARGKLHIFNAKKAAAHYKCMLFSSGTVQLTTTSRAEFGDFDIKTVLQEIKRGKRMVCSFYICYATLHLICCFKFRVHPKFIQSSENLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PHD finger protein 6
别名 BORJ, CENP-31, PHD-like zinc finger protein, PHF 6
计算分子量365aa,41 kDa; 312aa,35 kDa
观测分子量 41 kDa
GenBank蛋白编号BC005994
基因名称 PHF6
Gene ID (NCBI) 84295
RRIDAB_2165369
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWS0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PHF6 protein is highly conserved in vertebrates. Structurally, PHF6 protein contains two plant homeodomain (PHD)-like zinc fingers, two nuclear localization sequences and a nucleolar localization sequence, suggesting that PHF6 may play a role in the regulation of transcription. PHD zinc fingers are structurally conserved modules that interact with chromatin or mediate protein-protein interactions. PHF6 associates with subunits of the PAF1 transcription elongation complex including PAF1, LEO1, CTR9 and CDC73.The interaction of PHF6 with the PAF1 complex plays a critical role in PHF6 function in neuronal migration in the cerebral cortex.

实验方案

Product Specific Protocols
IF protocol for PHF6 antibody 51062-1-APDownload protocol
IHC protocol for PHF6 antibody 51062-1-APDownload protocol
WB protocol for PHF6 antibody 51062-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Leuk Res

MiR-1306-5p promotes cell proliferation and inhibits cell apoptosis in acute myeloid leukemia by downregulating PHF6 expression.

Authors - Xiang Gao
View Article
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