×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 51062-1-AP

    WB analysis using 51062-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 51062-1-AP (PHF6 antibody) at dilution of 1:64000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 51062-1-AP

    IHC staining of human ovary tumor using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51062-1-AP (PHF6 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IHC staining of human placenta using 51062-1-AP

    IHC staining of human placenta using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IHC staining of human placenta using 51062-1-AP

    IHC staining of human placenta using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IHC staining of human stomach cancer using 51062-1-AP

    IHC staining of human stomach cancer using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IHC staining of mouse brain using 51062-1-AP

    IHC staining of mouse brain using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IHC staining of mouse brain using 51062-1-AP

    IHC staining of mouse brain using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IHC staining of mouse stomach using 51062-1-AP

    IHC staining of mouse stomach using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IHC staining of rat brain using 51062-1-AP

    IHC staining of rat brain using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IHC staining of rat ovary using 51062-1-AP

    IHC staining of rat ovary using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IHC staining of rat stomach using 51062-1-AP

    IHC staining of rat stomach using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PHF6 antibody included
  • IF Staining of HeLa using 51062-1-AP

    IF Staining of HeLa using 51062-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PHF6 antibody (51062-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

PHF6 Polyclonal antibody

PHF6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 51062-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

BORJ, CENP-31, PHD-like zinc finger protein, PHF 6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman placenta tissue, human cervical cancer tissue, human stomach cancer tissue, rat brain tissue, rat stomach tissue, human ovary tumor tissue, mouse brain tissue, rat ovary tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PHF6 Ready-To-Use IHC Kit. PHF6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

51062-1-AP targets PHF6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag2102

Product name: Recombinant human PHF6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-312 aa of BC005994

Sequence: MSSSVEQKKGPTRQRKCGFCKSNRDKECGQLLISENQKVAAHHKCMLFSSALVSSHSDNESLGGFSIEDVQKEIKRGTKLMCSLCHCPGATIGCDVKTCHRTYHYHCALHDKAQIREKPSQGIYMAYCRKHKKTAHNSEAADLEESFNEHELEPSSPKSKKKSRKGRPRKTNFKGLSEDTRSTSSHGTDEMESSSYRDRSPHRSSPSDTRPKCGFCHVGEEENEARGKLHIFNAKKAAAHYKCMLFSSGTVQLTTTSRAEFGDFDIKTVLQEIKRGKRMVCSFYICYATLHLICCFKFRVHPKFIQSSENLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PHD finger protein 6
别名 BORJ, CENP-31, PHD-like zinc finger protein, PHF 6
计算分子量365aa,41 kDa; 312aa,35 kDa
观测分子量 41 kDa
GenBank蛋白编号BC005994
基因名称 PHF6
Gene ID (NCBI) 84295
RRIDAB_2165369
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWS0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PHF6 protein is highly conserved in vertebrates. Structurally, PHF6 protein contains two plant homeodomain (PHD)-like zinc fingers, two nuclear localization sequences and a nucleolar localization sequence, suggesting that PHF6 may play a role in the regulation of transcription. PHD zinc fingers are structurally conserved modules that interact with chromatin or mediate protein-protein interactions. PHF6 associates with subunits of the PAF1 transcription elongation complex including PAF1, LEO1, CTR9 and CDC73.The interaction of PHF6 with the PAF1 complex plays a critical role in PHF6 function in neuronal migration in the cerebral cortex.

实验方案

Product Specific Protocols
IF protocol for PHF6 antibody 51062-1-APDownload protocol
IHC protocol for PHF6 antibody 51062-1-APDownload protocol
WB protocol for PHF6 antibody 51062-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Leuk Res

MiR-1306-5p promotes cell proliferation and inhibits cell apoptosis in acute myeloid leukemia by downregulating PHF6 expression.

Authors - Xiang Gao
View Article
||
New chat

Able™

正在加载，请稍候...