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PHF5A Monoclonal antibody

PHF5A Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G7E2

Cat No : 68196-1-Ig

Print datasheet

Synonyms

bK223H9.2, INI, PHD finger protein 5A, PHF5A, SF3b14b

验证数据展示

  • WB analysis using 68196-1-Ig

    WB analysis using 68196-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68196-1-Ig (PHF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy PHF5A Ready-To-Use IHC Kit. PHF5A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68196-1-Ig targets PHF5A in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA
Tested Reactivity Human, mouse
Immunogen PHF5A fusion protein Ag7804 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name PHD finger protein 5A
Synonyms bK223H9.2, INI, PHD finger protein 5A, PHF5A, SF3b14b
Calculated Molecular Weight 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberBC007321
Gene Symbol PHF5A
Gene ID (NCBI) 84844
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ7RTV0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The PHD finger-like domain protein 5a (PHF5A) is ubiquitously expressed and is located in the nucleus. The gene Phf5a and its human counterpart PHF5A (former name Ini) are located on rat chromosome 7 (RNO7q34) and human chromosome 22 (HSA2213q2), respectively. The rat gene consists of four exons, while the human gene have five exons. Both genes encode a highly conserved protein of 110 amino acids that contains a PHD finger domain.(PMID: 23675859) It acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER-alpha and is involved in pre-mRNA splicing.

实验方案

Product Specific Protocols
WB protocol for PHF5A antibody 68196-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols