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验证数据展示

  • WB analysis using 68196-1-Ig

    WB analysis using 68196-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68196-1-Ig (PHF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

PHF5A Monoclonal antibody

PHF5A Monoclonal Antibody for WB, ELISA
Cat No. 68196-1-Ig

产品说明书

CloneNo. 1G7E2

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

WB, ELISA

别名

bK223H9.2, INI, PHD finger protein 5A, PHF5A, SF3b14b

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy PHF5A Ready-To-Use IHC Kit. PHF5A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68196-1-Ig targets PHF5A in WB, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag7804

Product name: Recombinant human INI-1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-110 aa of BC007321

Sequence: MAKHHPDLIFCRKQAGVAIGRLCEKCDGKCVICDSYVRPCTLVRICDECNYGSYQGRCVICGGPGVSDAYYCKECTIQEKDRDGCPKIVNLGSSKTDLFYERKKYGFKKR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 PHD finger protein 5A
别名 bK223H9.2, INI, PHD finger protein 5A, PHF5A, SF3b14b
计算分子量 12 kDa
观测分子量12 kDa
GenBank蛋白编号BC007321
基因名称 PHF5A
Gene ID (NCBI) 84844
RRIDAB_2935285
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ7RTV0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The PHD finger-like domain protein 5a (PHF5A) is ubiquitously expressed and is located in the nucleus. The gene Phf5a and its human counterpart PHF5A (former name Ini) are located on rat chromosome 7 (RNO7q34) and human chromosome 22 (HSA2213q2), respectively. The rat gene consists of four exons, while the human gene have five exons. Both genes encode a highly conserved protein of 110 amino acids that contains a PHD finger domain.(PMID: 23675859) It acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER-alpha and is involved in pre-mRNA splicing.

实验方案

Product Specific Protocols
WB protocol for PHF5A antibody 68196-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols