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验证数据展示

  • WB analysis using 25862-1-AP

    WB analysis using 25862-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25862-1-AP (PHF23 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 25862-1-AP

    WB analysis of HeLa using 25862-1-AP

    WB result of PHF23 antibody (25862-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHF23 transfected HeLa cells.

  • IF Staining of A549 using 25862-1-AP

    IF Staining of A549 using 25862-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using PHF23 antibody (25862-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

PHF23 Polyclonal antibody

PHF23 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25862-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

PDH-containing protein JUNE-1, hJUNE-1b, hJUNE 1b

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25862-1-AP targets PHF23 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 PHF23 fusion protein Ag23044 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PHD finger protein 23
别名 PDH-containing protein JUNE-1, hJUNE-1b, hJUNE 1b
计算分子量 403 aa, 44 kDa
观测分子量~65 kDa
GenBank蛋白编号BC002509
基因名称 PHF23
Gene ID (NCBI) 79142
RRIDAB_3085824
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BUL5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PHD finger protein 23 (PHF23), also known as hJUNE-1b, belongs to the PHF23 family. As a putative epigenetic reader, the PHD domain of PHF23 specifically binds to tri-methylated lysine 4 of histone H3 (H3K4me3). PHF23 is widely expressed in human tissues and various cell lines and exerts a negative effect on autophagy by promoting the degradation of E3 ligase LRSAM1 (leucine-rich repeat and sterile alpha motif containing 1) (PMID: 32978226, 32380078). The observed molecular weight of PHF23 is ~65 kDa, which may be caused by post-translational modification. The specificity of this antibody was demonstrated by siRNA-mediated knockdown of target protein.

实验方案

Product Specific Protocols
WB protocol for PHF23 antibody 25862-1-APDownload protocol
IF protocol for PHF23 antibody 25862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols