验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue |
| Positive IF/ICC detected in | Mouse ES cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 5 publications below |
| WB | See 9 publications below |
| IHC | See 2 publications below |
产品信息
11895-1-AP targets PHF19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2511 Product name: Recombinant human PHF19 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-207 aa of BC022374 Sequence: MENRALDPGTRDSYGATSHLPNKGALAKVKNNFKDLMSKLTEGQYVLCRWTDGLYYLGKIKRVSSSKQSCLVTFEDNSKYWVLWKDIQHAGVPGEEPKCNICLGKTSGPLNEILICGKCGLGYHQQCHIPIAGSADQPLLTPWFCRRCIFALAVRVSLPSSPVPASPASSSGADQRLPSQSLSSKQKGHTWALETDSASATVLGQDL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PHD finger protein 19 |
| 别名 | hPCL3, MTF2L1, PCL3, PHD finger protein 19, Polycomb like protein 3 |
| 计算分子量 | 580 aa, 63 kDa |
| 观测分子量 | 22 kDa, 66 kDa |
| GenBank蛋白编号 | BC022374 |
| 基因名称 | PHF19 |
| Gene ID (NCBI) | 26147 |
| RRID | AB_10804890 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5T6S3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PHD FINGER PROTEIN 19(PHF19), a protein homologous to the Drosophila 'Polycomblike' (Pcl) protein, encoded by human gene PCL, which encodes 2 deduced proteins: a short form of 207 aa and a long form of 580 aa. The PCL family proteins contains a PCL homology domain, a Tudor domain, 2 PHD fingers and a C-terminal tail. Both of these forms can inhibit transcriptional activity. And the long form is corelated with cancer. The mRNA level of PHF19 is low in many normal tissue, it's elevated in cancer-derived cell lines.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PHF19 antibody 11895-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Dissecting the role of H3K27 acetylation and methylation in PRC2 mediated control of cellular identity. | ||
Mol Cell An H3K36 methylation-engaging Tudor motif of polycomb-like proteins mediates PRC2 complex targeting.
| ||
Mol Cell PALI1 promotes tumor growth through competitive recruitment of PRC2 to G9A-target chromatin for dual epigenetic silencing | ||
Front Immunol Comprehensive Pan-Cancer Genomic Analysis Reveals PHF19 as a Carcinogenic Indicator Related to Immune Infiltration and Prognosis of Hepatocellular Carcinoma. | ||
Brain Res Bull PDGF-BB mitigates pericyte injury by activating the PHF19-PRC2 complex via the miR-221/BRCA1 signaling axis in Alzheimer's disease. | ||
Pathology High PHD finger protein 19 expression predicts inferior prognosis in diffuse large B-cell lymphoma |
