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PHF14 Polyclonal antibody
PHF14 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IHC, IP, WB, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, MDA-MB-453s cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PHF14 Ready-To-Use IHC Kit. PHF14 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 24787-1-AP is PHF14 Fusion Protein expressed in E. coli.
| Tested Applications | IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC, CoIP, ChIP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | PHF14 fusion protein Ag20542 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | PHD finger protein 14 |
| Synonyms | KIAA0783, PHD finger protein 14, PHF14 |
| Calculated Molecular Weight | 888 aa, 100 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | BC152414 |
| Gene Symbol | PHF14 |
| Gene ID (NCBI) | 9678 |
| RRID | AB_2879724 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94880 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PHF14 also named as KIAA0783 or PHD finger protein 14 is 888 amino acid protein, which contains 2 PHD-type znic fingers. PHF14 localizes in nucleus and may be involved in the regulation of transcription, DNA dependent. The function of PHF14 is still non-known.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PHF14 antibody 24787-1-AP | Download protocol |
| IHC protocol for PHF14 antibody 24787-1-AP | Download protocol |
| IP protocol for PHF14 antibody 24787-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics | ||
Elife Integrated analysis of H2A.Z isoforms function reveals a complex interplay in gene regulation. | ||
Biomed Res Int PHF14 Promotes Cell Proliferation and Migration through the AKT and ERK1/2 Pathways in Gastric Cancer Cells.
| ||
Nucleic Acids Res The high mobility group protein HMG20A cooperates with the histone reader PHF14 to modulate TGFβ and Hippo pathways
| ||
Cell Discov PHF14 enhances DNA methylation of SMAD7 gene to promote TGF-β-driven lung adenocarcinoma metastasis | ||
Oncotarget Pulsed SILAC-based proteomic analysis unveils hypoxia- and serum starvation-induced de novo protein synthesis with PHD finger protein 14 (PHF14) as a hypoxia sensitive epigenetic regulator in cell cycle progression.
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