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PHF12 Polyclonal antibody

PHF12 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 24485-1-AP

Print datasheet

Synonyms

KIAA1523, PF1, PHD factor 1, PHD finger protein 12, PHF12

验证数据展示

  • WB analysis of mouse brain using 24485-1-AP

    WB analysis of mouse brain using 24485-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24485-1-AP (PHF12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 24485-1-AP

    IP experiment of mouse brain using 24485-1-AP

    IP result of anti-PHF12 (IP:24485-1-AP, 4ug; Detection:24485-1-AP 1:500) with mouse brain tissue lysate 3600ug.

经过测试的应用

Positive WB detected inmouse brain tissue, mouse lung tissue
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 24485-1-AP is PHF12 Fusion Protein expressed in E. coli.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen PHF12 fusion protein Ag18471 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PHD finger protein 12
Synonyms KIAA1523, PF1, PHD factor 1, PHD finger protein 12, PHF12
Calculated Molecular Weight 1004 aa, 110 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberBC121043
Gene Symbol PHF12
Gene ID (NCBI) 57649
RRIDAB_2879566
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QT6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Plant homeodomain [PHD] zinc finger protein 12 (PHF12), also known Pf1, is a member of the PHD zinc finger family of proteins. Pf1 associates with a chromatin-interacting protein complex comprised of MRG15, Sin3B, and histone deacetylase 1 (HDAC1) that functions as a transcriptional modulator.

实验方案

Product Specific Protocols
WB protocol for PHF12 antibody 24485-1-APDownload protocol
IP protocol for PHF12 antibody 24485-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO Rep

MYC promotes cancer progression by modulating m6 A modifications to suppress target gene translation.

Authors - Gongwei Wu
View Article