验证数据展示
产品信息
66934-1-PBS targets PHEX in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.
| 经测试应用 | WB, IHC, Indirect ELISA Application Description |
| 经测试反应性 | human, rat, pig |
| 免疫原 |
CatNo: Ag20290 Product name: Recombinant human PHEX protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 41-391 aa of BC105059 Sequence: SQGLLSLQAKQEYCLKPECIEAAAAILSKVNLSVDPCDNFFRFACDGWISNNPIPEDMPSYGVYPWLRHNVDLKLKELLEKSISRRRDTEAIQKAKILYSSCMNEKAIEKADAKPLLHILRHSPFRWPVLESNIGPEGVWSERKFSLLQTLATFRGQYSNSVFIRLYVSPDDKASNEHILKLDQATLSLAVREDYLDNSTEAKSYRDALYKFMVDTAVLLGANSSRAEHDMKSVLRLEIKIAEIMIPHENRTSEAMYNKMNISELSAMIPQFDWLGYIKKVIDTRLYPHLKDISPSENVVVRVPQYFKDLFRILGSERKKTIANYLVWRMVYSRIPNLSRRFQYRWLEFSR 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | phosphate regulating endopeptidase homolog, X-linked |
| 别名 | 1D4B2, EC:3.4.24.-, HPDR, HYP1, PEX |
| 计算分子量 | 749 aa, 86 kDa |
| 观测分子量 | 76-80 kDa, 95-100 kDa |
| GenBank蛋白编号 | BC105059 |
| 基因名称 | PHEX |
| Gene ID (NCBI) | 5251 |
| RRID | AB_2882259 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P78562 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
PHEX encodes a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein usually expressed in osteocytes, osteoblasts and odontoblasts, and it was thought to be involved in bone and dentin mineralization and renal phosphate reabsorption (PMID:28982589). Otherwise, studies showed that PHEX transcripts were identified in human osteosarcoma-derived cells and in differentiated mouse osteoblasts, but not in immature mouse preosteoblasts, indicating the stage-specific expression of the protein (PMID:9199999). Defects in PHEX are responsible for X-linked hypophosphatemic rickets (HYP) (PMID:22339660). Glycosylation of proteins results in higher molecular weight, and about 95-100 kDa band were reported (PMID:25128529; PMID:18728349).
