验证数据展示
经过测试的应用
| Positive WB detected in | CHO cells, ROS1728 cells, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
30668-1-AP targets PHEX in WB, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat, hamster |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag33428 Product name: Recombinant human PHEX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 430-641 aa of BC105059 Sequence: DKKEMMEELVEGVRWAFIDMLEKENEWMDAGTKRKAKEKARAVLAKVGYPEFIMNDTHVNEDLKAIKFSEADYFGNVLQTRKYLAQSDFFWLRKAVPKTEWFTNPTTVNAFYSASTNQIRFPAGELQKPFFWGTEYPRSLSYGAIGVIVGHEFTHGFDNNGRKYDKNGNLDPWWSTESEEKFKEKTKCMINQYSNYYWKKAGLNVKGKRTLG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphate regulating endopeptidase homolog, X-linked |
| 别名 | EC:3.4.24.-, HPDR, HYP1, PEX, Phosphate-regulating neutral endopeptidase PHEX |
| 计算分子量 | 749 aa, 86 kDa |
| 观测分子量 | 76-80 kDa, 95-100 kDa |
| GenBank蛋白编号 | BC105059 |
| 基因名称 | PHEX |
| Gene ID (NCBI) | 5251 |
| RRID | AB_3742364 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P78562 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Phex encodes a 100- to 105-kDa transmembrane glycoprotein, which is present in bones and teeth of normal mice but not Hyp animals. Phex protein expression in femur and calvaria decreases with age, high levels of Phex detected in osteocytes make this protein an interesting marker to study the later stages of osteoblastic cell differentiation (PMID: 10934642). The 76 kDa represents the deglycosylated core-glycosylated forms within the ERs, the 95 kDa represents the core-glycosylated and unmatured form within the ERs, the 105 kDa species of PHEX protein represents the fully processed, matured for(PMID: 3251189, 35654784).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PHEX antibody 30668-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
