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PHC2 Polyclonal antibody

PHC2 Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 12867-1-AP

Print datasheet

Synonyms

EDR2, HPH2, PH2, PHC2, Polyhomeotic like protein 2

验证数据展示

  • IP experiment of HL-60 using 12867-1-AP

    IP experiment of HL-60 using 12867-1-AP

    IP result of anti-PHC2 (IP:12867-1-AP, 4ug; Detection:12867-1-AP 1:500) with HL-60 cells lysate 4000 ug.

  • WB analysis of A375 using 12867-1-AP

    WB analysis of A375 using 12867-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12867-1-AP (PHC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 12867-1-AP

    IF Staining of A375 using 12867-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 12867-1-AP (PHC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA375 cells, A549 cells
Positive IP detected inHL-60 cells
Positive IF detected inA375 cells
Planning an IHC experiment? We recommend our IHCeasy PHC2 Ready-To-Use IHC Kit. PHC2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12867-1-AP targets PHC2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen PHC2 fusion protein Ag3507 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polyhomeotic homolog 2 (Drosophila)
Synonyms EDR2, HPH2, PH2, PHC2, Polyhomeotic like protein 2
Calculated Molecular Weight 858 aa, 91 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC028396
Gene Symbol PHC2
Gene ID (NCBI) 1912
RRIDAB_2877890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IXK0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PHC2 antibody 12867-1-APDownload protocol
IF protocol for PHC2 antibody 12867-1-APDownload protocol
IP protocol for PHC2 antibody 12867-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols