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KD/KO Validated

Prohibitin 2 Polyclonal antibody

Prohibitin 2 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

FC, IF/ICC, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Publications

Cat No : 12295-1-AP

Print datasheet

Synonyms

BAP, Bap37, D prohibitin, p22, PHB2, PNAS 141, prohibitin 2, REA

验证数据展示

WB analysis using 12295-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12295-1-AP (Prohibitin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 12295-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12295-1-AP (Prohibitin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
WB analysis of HeLa using 12295-1-AP
WB result of Prohibitin 2 antibody (12295-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Prohibitin 2 transfected HeLa cells.
IP experiment of HeLa using 12295-1-AP
IP result of anti-Prohibitin 2 (IP:12295-1-AP, 3ug; Detection:12295-1-AP 1:1000) with HeLa cells lysate 1840ug.
IHC staining of human heart using 12295-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 10x lens).
Ready-to-use IHC kits available with PHB2 antibody included
IHC staining of human heart using 12295-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 40x lens).
Ready-to-use IHC kits available with PHB2 antibody included
FC experiment of HeLa using 12295-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Prohibitin 2 (12295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 12295-1-AP

Size

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经过测试的应用

Positive WB detected in	HeLa cells
Positive IP detected in	HeLa cells, mouse brain tissue
Positive IHC detected in	human heart tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells
Positive FC detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy PHB2 Ready-To-Use IHC Kit. PHB2 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 8 publications below
WB	See 36 publications below
IHC	See 3 publications below
IF	See 8 publications below
IP	See 2 publications below
CoIP	See 2 publications below

产品信息

12295-1-AP targets Prohibitin 2 in WB, IP, IF, FC, IHC, CoIP, ELISA, Electrochemical Immunosensor applications and shows reactivity with human, mouse samples.

Tested Applications	FC, IF/ICC, IHC, IP, WB, ELISA
Cited Applications	WB, IP, IF, IHC, CoIP, Electrochemical Immunosensor
Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, rice
Immunogen	Prohibitin 2 fusion protein Ag2977 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	prohibitin 2
Synonyms	BAP, Bap37, D prohibitin, p22, PHB2, PNAS 141, prohibitin 2, REA
Calculated Molecular Weight	299 aa, 33 kDa
Observed Molecular Weight	33 kDa
GenBank Accession Number	BC014766
Gene Symbol	PHB2
Gene ID (NCBI)	11331
RRID	AB_2164779
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99623
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

PHB2, also named as BAP, REA, D-prohibitin, BAP37 and Prohibitin-2, belongs to the prohibitin family. It acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. PHB2 functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. PHB2 is probably involved in regulating mitochondrial respiration activity and in aging.(PMID: 10359819)

实验方案

Product Specific Protocols
WB protocol for Prohibitin 2 antibody 12295-1-AP	Download protocol
IHC protocol for Prohibitin 2 antibody 12295-1-AP	Download protocol
IF protocol for Prohibitin 2 antibody 12295-1-AP	Download protocol
IP protocol for Prohibitin 2 antibody 12295-1-AP	Download protocol
FC protocol for Prohibitin 2 antibody 12295-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria Authors - Sabine Poerschke View Article
human	WB	J Am Soc Nephrol Bif-1 Interacts with Prohibitin-2 to Regulate Mitochondrial Inner Membrane during Cell Stress and Apoptosis. Authors - Sung-Gyu Cho View Article
mouse	WB	Neuron Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2. Authors - Kelly A Chamberlain View Article
rat	IF	Nat Commun Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination. Authors - Y Poitelon View Article
mouse		Redox Biol PHB2 ameliorates Doxorubicin-induced cardiomyopathy through interaction with NDUFV2 and restoration of mitochondrial complex I function Authors - Mingjie Yang View Article
human	IF	J Exp Clin Cancer Res PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer Authors - Liang Xu View Article

Prohibitin 2 Polyclonal antibody

Prohibitin 2 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

验证数据展示

WB analysis using 12295-1-AP

IF Staining of HepG2 using 12295-1-AP

WB analysis of HeLa using 12295-1-AP

IP experiment of HeLa using 12295-1-AP

IHC staining of human heart using 12295-1-AP

IHC staining of human heart using 12295-1-AP

FC experiment of HeLa using 12295-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria

Bif-1 Interacts with Prohibitin-2 to Regulate Mitochondrial Inner Membrane during Cell Stress and Apoptosis.

Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2.

Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.

PHB2 ameliorates Doxorubicin-induced cardiomyopathy through interaction with NDUFV2 and restoration of mitochondrial complex I function

PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer

相关产品

Flow Cytometry Perm Buffer (10X)