验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, mouse heart tissue |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83302-1-RR targets PGT in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag5594 Product name: Recombinant human PGT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 422-643 aa of BC041140 Sequence: TPTVAEVYPPSTSSSIHPQSPACRRDCSCPDSIFHPVCGDNGIEYLSPCHAGCSNINMSSATSKQLIYLNCSCVTGGSASAKTGSCPVPCAHFLLPAIFLISFVSLIACISHNPLYMMVLRVVNQEEKSFAIGVQFLLMRLLAWLPSPALYGLTIDHSCIRWNSLCLGRRGACAYYDNDALRDRYLGLQMGYKALGMLLLCFISWRVKKNKEYNVQKAAGLI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | solute carrier organic anion transporter family, member 2A1 |
| 别名 | SLCO2A1, 230139F5, OATP2A1, PHOAR2 |
| 计算分子量 | 70 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC041140 |
| 基因名称 | PGT |
| Gene ID (NCBI) | 6578 |
| RRID | AB_3670971 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q92959 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Prostaglandin transporter (PGT), also known as SLCO2A1, has been identified as a functional prostaglandin (PG) carrier that mediates either the release of newly-synthesized PGs by cells and/or the uptake of PGs prior to intracellular oxidation. Northern blot analysis revealed that PGT mRNA has broad tissue distribution, suggesting its role in the transport and/ or metabolic clearance of PG in diverse tissues (PMID: 8787677,22331663).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PGT antibody 83302-1-RR | Download protocol |
| WB protocol for PGT antibody 83302-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
