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验证数据展示

  • WB analysis using 60249-1-Ig (same clone as 60249-1-PBS)

    WB analysis using 60249-1-Ig (same clone as 60249-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60249-1-Ig (PGRMC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    IHC staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60249-1-Ig (PGRMC2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGRMC2 antibody included
  • IHC staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    IHC staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60249-1-Ig (PGRMC2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGRMC2 antibody included
  • IF Staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    IF Staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using PGRMC2 antibody (60249-1-Ig, Clone: 5F10D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

  • IF Staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    IF Staining of human cervical cancer using 60249-1-Ig (same clone as 60249-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using PGRMC2 antibody (60249-1-Ig, Clone: 5F10D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 60249-1-Ig (same clone as 60249-1-PBS)

    FC experiment of HepG2 using 60249-1-Ig (same clone as 60249-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PGRMC2 (60249-1-Ig, Clone:5F10D7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 60249-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PGRMC2 Monoclonal antibody, PBS Only

PGRMC2 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 60249-1-PBS

产品说明书

CloneNo. 5F10D7

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig

应用

WB, IHC, IF-P, FC (Intra), Indirect ELISA

别名

5F10D7, DG6, Membrane-associated progesterone receptor component 2, PMBP

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PGRMC2 Ready-To-Use IHC Kit. PGRMC2 primary antibody included.

产品信息

60249-1-PBS targets PGRMC2 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag20077

Product name: Recombinant human PGRMC2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 55-145 aa of BC016692

Sequence: LLNVALVALVLLGAYRLWVRWGRRGLGAGAGAGEESPATSLPRMKKRDFSLEQLRQYDGSRNPRILLAVNGKVFDVTKGSKFYGPAGPYGI

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 progesterone receptor membrane component 2
别名 5F10D7, DG6, Membrane-associated progesterone receptor component 2, PMBP
计算分子量 223 aa, 24 kDa
观测分子量 24 kDa, 28 kDa
GenBank蛋白编号BC016692
基因名称 PGRMC2
Gene ID (NCBI) 10424
RRIDAB_2881370
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO15173
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Membrane-associated progesterone receptor component 2 (PGRMC2), also known as DG6 or PMBP, belongs to the cytochrome b5 family. This protein might play a role in cancer. The gene of PGRMC2 maps to chromosome 4q26, and encodes a 223-amino acid single-pass membrane protein with a cytochrome b5 heme-binding domain in its cytoplasmic domain. PGRMC2 has a calculated molecular weight of 24 kDa. The band of about 28 kDa detected by this monoclonal antibody is unknown but may represent phosphorylated form of PGRMC2 (PMID: 28104494).

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