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验证数据展示

  • WB analysis using 11022-1-AP

    WB analysis using 11022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11022-1-AP (PGM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 11022-1-AP

    IP experiment of HEK-293 using 11022-1-AP

    IP result of anti-PGM2 (IP:11022-1-AP, 4ug; Detection:11022-1-AP 1:200) with HEK-293 cells lysate 2400 ug.

  • IHC staining of human pancreas cancer using 11022-1-AP

    IHC staining of human pancreas cancer using 11022-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11022-1-AP (PGM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 11022-1-AP

    IHC staining of human pancreas cancer using 11022-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11022-1-AP (PGM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

PGM2 Polyclonal antibody

PGM2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11022-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA and More (1)

别名

Glucose phosphomutase 2, MSTP006, PGM 2, PGM2, Phosphodeoxyribomutase, phosphoglucomutase 2, Phosphopentomutase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, human brain tissue, fetal human brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11022-1-AP targets PGM2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag1488

Product name: Recombinant human PGM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 264-612 aa of BC010087

Sequence: QSAFKAFDLVPPEAVPEQKDPDPEFPTVKYPNPEEGKGVLTLSFALADKTKARIVLANDPDADRLAVAEKQDSGEWRVFSGNELGALLGWWLFTSWKEKNQDRSALKDTYMLSSTVSSKILRAIALKEGFHFEETLTGFKWMGNRAKQLIDQGKTVLFAFEEAIGYMCCPFVLDKDGVSAAVISAELASFLATKNLSLSQQLKAIYVEYGYHITKASYFICHDQETIKKLFENLRNYDGKNNYPKACGKFEISAIRDLTTGYDDSQPDKKAVLPTSKSSQMITFTFANGGVATMRTSGTEPKIKYYAELCAPPGNSDPEQLKKELNELVSAIEEHFFQPQKYNLQPKAD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoglucomutase 2
别名 Glucose phosphomutase 2, MSTP006, PGM 2, PGM2, Phosphodeoxyribomutase, phosphoglucomutase 2, Phosphopentomutase
计算分子量 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC010087
基因名称 PGM2
Gene ID (NCBI) 55276
RRIDAB_2161422
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96G03
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PGM2 antibody 11022-1-APDownload protocol
IHC protocol for PGM2 antibody 11022-1-APDownload protocol
IP protocol for PGM2 antibody 11022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Metab

Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions

Authors - Owen S Skinner
  • KO Validated
View Article
humanWB,IF

Heliyon

Nuclear translocation of nucleotide enzyme Phosphoglucomutase 2 governs DNA damage response and anti-tumor immunity

Authors - Yingying Lyu
View Article