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验证数据展示

  • WB analysis using 87478-1-RR (same clone as 87478-1-PBS)

    WB analysis using 87478-1-RR (same clone as 87478-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87478-1-RR (PGK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87478-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 87478-1-RR (same clone as 87478-1-PBS)

    FC experiment of HeLa using 87478-1-RR (same clone as 87478-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PGK1 Recombinant monoclonal antibody (87478-1-RR, Clone:252909A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 87478-1-PBS in a different storage buffer formulation.

PGK1 Recombinant monoclonal antibody, PBS Only

PGK1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 87478-1-PBS

产品说明书

CloneNo. 252909A5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Indirect ELISA

别名

Cell migration-inducing gene 10 protein, EC:2.7.2.3, OK/SW-cl.110, PGKA, PRP 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PGK1 Ready-To-Use IHC Kit. PGK1 primary antibody included.

产品信息

87478-1-PBS targets PGK1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12119

Product name: Recombinant human PGK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-417 aa of BC103752

Sequence: MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphoglycerate kinase 1
别名 Cell migration-inducing gene 10 protein, EC:2.7.2.3, OK/SW-cl.110, PGKA, PRP 2
计算分子量 417 aa, 45 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC103752
基因名称 PGK1
Gene ID (NCBI) 5230
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP00558
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PGK1, also known as PGKA, belongs to the phosphoglycerate kinase family. PGK1 is the first ATP-generating enzyme in glycolysis, which catalyzes the conversion of 1,3-diphosphoglycerate (1,3-BPG) to 3-phosphoglycerate (3-PG) and produces one molecule of ATP. PGK1 has other functions besides regulating glycolytic metabolism, including mediating autophagy initiation, DNA replication and repair in mammal cell nuclei (PMID: 31815035, PMID: 31911580).

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