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验证数据展示

  • WB analysis of human stomach using 16881-1-AP

    WB analysis of human stomach using 16881-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 16881-1-AP (Pepsinogen II antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 16881-1-AP

    IHC staining of human stomach cancer using 16881-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16881-1-AP (Pepsinogen II antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Pepsinogen II Polyclonal antibody

Pepsinogen II Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16881-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

PGC, PGⅡ, EC:3.4.23.3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman stomach tissue, mouse stomach tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16881-1-AP targets Pepsinogen II in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Pepsinogen II fusion protein Ag10332 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 progastricsin (pepsinogen C)
别名 PGC, PGⅡ, EC:3.4.23.3
计算分子量 388 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC073740
基因名称 PGC
Gene ID (NCBI) 5225
RRIDAB_10596919
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20142
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pepsinogen II also known as progastricsin or PGC(pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for Pepsinogen II antibody 16881-1-APDownload protocol
IHC protocol for Pepsinogen II antibody 16881-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols