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验证数据展示

  • WB analysis of human stomach using 16881-1-AP

    WB analysis of human stomach using 16881-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 16881-1-AP (Pepsinogen II antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 16881-1-AP

    IHC staining of human stomach cancer using 16881-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16881-1-AP (Pepsinogen II antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Pepsinogen II Polyclonal antibody

Pepsinogen II Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16881-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

PGC, PGⅡ, EC:3.4.23.3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman stomach tissue, mouse stomach tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16881-1-AP targets Pepsinogen II in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag10332

Product name: Recombinant human PGC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-388 aa of BC073740

Sequence: MKWMVVVLVCLQLLEAAVVKVPLKKFKSIRETMKEKGLLGEFLRTHKYDPAWKYRFGDLSVTYEPMAYMDAAYFGEISIGTPPQNFLVLFDTGSSNLWVPSVYCQSQACTSHSRFNPSESSTYSTNGQTFSLQYGSGSLTGFFGYDTLTVQSIQVPNQEFGLSENEPGTNFVYAQFDGIMGLAYPALSVDEATTAMQGMVQEGALTSPVFSVYLSNQQGSSGGAVVFGGVDSSLYTGQIYWAPVTQELYWQIGIEEFLIGGQASGWCSEGCQAIVDTGTSLLTVPQQYMSALLQATGAQEDEYGQFLVNCNSIQNLPSLTFIINGVEFPLPPSSYILSNNGYCTVGVEPTYLSSQNGQPLWILGDVFLRSYYSVYDLGNNRVGFATAA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 progastricsin (pepsinogen C)
别名 PGC, PGⅡ, EC:3.4.23.3
计算分子量 388 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC073740
基因名称 PGC
Gene ID (NCBI) 5225
RRIDAB_10596919
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20142
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pepsinogen II also known as progastricsin or PGC(pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for Pepsinogen II antibody 16881-1-APDownload protocol
IHC protocol for Pepsinogen II antibody 16881-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols